second quarter 2023 financial results and business update conference call for Yield10 Bioscience. During the call, participants will be in a listen-only mode. The presenters will address questions from analysts today. If anyone should require operator assistance during the conference, please press star 0 on your telephone keypad. As a reminder, this conference is being recorded. I would now like to turn the conference over to your host, Yield10 Vice President of Planning and Corporate Communications, Lynne Brum.
Thank you, Melissa. Good afternoon, everyone. Welcome to the Yield10 Bioscience second quarter 2023 conference call. Joining me on the call today are President and CEO, Dr. Oliver Peoples, Vice President of Research and Chief Science Officer, Dr. Kristi Snell, and Chief Accounting Officer, Chuck Haaser. Earlier this afternoon, Yield10 issued our second quarter 2023 financial results. This press release, as well as slides that accompany today's presentation, are available on the Investor Relations Events section of our website at yield10bio.com. Let's turn to slide 2. Please note that as part of our discussion today, management will be making forward-looking statements. These statements are not guarantees of future performance, and therefore, you should not place undue reliance on them. Investors are also cautioned that statements that are not strictly historical constitute forward-looking statements.
Such forward-looking statements are subject to a number of risks and uncertainties that could cause the actual results to differ materially from those anticipated. These risks include risks and uncertainties detailed in Yield10's filings with the SEC. The company undertakes no obligation to update any forward-looking statements in order to reflect events or circumstances that may arise after the date of this conference call. I'll turn the call over to Oli.
Thanks, Lynn. Good, good afternoon, everyone, thanks for joining our call. Let's turn to slide 3. I'm pleased to report that we are making solid progress across the business as we operationalize our commercial plan to launch Camelina as a platform crop, with a focus on biofuel feedstock markets and high-value market for omega-3 oils. Today, we will provide an update on our business, including recent accomplishments, the market opportunity and trends driving the biofuel market, our progress developing herbicide-tolerant Camelina varieties, our plan to scale up EPA omega-3 Camelina, we present second quarter financials and summarize key milestones. We will open the call to questions. Let's turn to slide 4 for progress on key 2023 business goals. Our main goal in 2023 is to establish and demonstrate the value chain from Yield10 seed to commercial offtake products.
I'm pleased to report that based on recent activities in the business, we are right on track executing against this goal. The value chain for Camelina in the biofuels market starts with seed genetics and quality seed production. The next step is building relationships with growers in the target growing regions and signing up to grow Camelina under contract. The third step is to line up grain offtake customers to buy the Camelina grain for their crushing and biofuel operations. We have now demonstrated each of the steps in this value chain, starting with the winter Camelina we contracted last year. We signed contracts and guided the growers through planting and harvest. For one farm located in southern Alberta, we directed delivery of the harvested Camelina to a regional biofuel partner, where the first crush of the Camelina grain has occurred, and conversion to biofuel is imminent.
The photos on the right side of the slide show the harvest of Winter Camelina grown in Southern Alberta and the delivery of the Camelina grain to an offtake customer. This represents a key milestone for Yield10, demonstrating the full value chain. On the business development side, our team continues to engage with potential supply chain partners, including ongoing discussions with Marathon Petroleum and Mitsubishi, for investment and offtake agreements for Camelina production in specified growing regions. Outside of the scope of these discussions, we have continued to respond to additional inbound inquiries from energy company partner prospects, with the goal of creating the option to establish a network of alliances to supply Camelina feedstock oil to the biofuel market. Let's now turn to slide 5: Camelina as a platform crop.
Our business plan is based on leveraging Camelina as a platform crop for producing oil, meal, and specialty products. We believe that by leveraging the demand created by biofuels and our advanced technology capabilities, we can transition Camelina from a niche crop into a major crop, planting on more than 30 million acres a year in North America. This won't be achieved overnight, but there are key attributes of Camelina that could drive the ramp-up in acres. For example, Camelina can be planted, harvested, stored, and processed on existing equipment on farm and at the processors. Next, another big driver adoption is that we are bringing forward not only spring varieties, but winter varieties of Camelina. Our winter varieties are proving to be extremely cold-tolerant and drought-resistant. With time, winter Camelina has potential for seamless integration into crop rotations with corn and soy.
Here, we would plant Camelina as a winter cover crop in the fall after corn harvest, and harvest late spring before planting soybean. I'll mention that in June, the USDA stepped up and indicated that they are offering crop insurance for Camelina in many states. Let's now turn to slide 6, the business model and markets for Camelina oil and meal. We are using a closed-loop contracted grain production model to produce Camelina oil for the biofuel market. The Camelina grain is comprised of about 35%-40% oil, and the protein meal, about 50%+5%, and basically, the rest of the seed. This is the co-product from oil extraction. Camelina oil has captured the attention of the renewable energy space because of its attractive composition and profile.
It is a very low carbon intensity, and its fatty acid profile is well suited for making renewable diesel and SAF. Camelina protein meal is approved for use in animal feed, representing an essential market and revenue stream for the grain. Now let's turn to slide 7, the biofuel opportunity. There's tremendous opportunity in the biofuel market, where vegetable oil is processed into sustainable aviation fuel, SAF, and renewable diesel. New demand is being generated as new refineries are coming online, and as new environmental commitments are being made to utilize these cleaner fuels across the globe. Biofuels players face two major sources of risk: volatility in pricing and availability of feedstock oils, and volatility in the regulatory environment, which, among other things, can impact the incentives for biofuel adoption.
For example, we are already seeing states like Illinois place limits on the amount of soybean oil that can be used for SAF, above which the tax breaks no longer apply. Camelina cultivation could address key aspects of risk for biofuel producers by expanding the overall supply of feedstock oil, especially using winter Camelina. Growing Camelina on otherwise fallow land, either winter or summer, can increase both feedstock oil for fuel and brings with it a new source of high-protein meal. Let's now turn to slide 8, establishing production and our early varieties. Our team has developed spring and winter Camelina varieties that have enabled us to establish production in the value chain. WDH2, shown in the center of the slide, is a winter variety with robust cold tolerance. WDH3 is an early-maturing winter variety, and E3902 is our genome-edited spring variety.
The development of these three varieties enabled Yield10 to begin the commercial launch with proprietary seed genetics, while we accelerated the development of our advanced varieties with elite herbicide technology for commercial launch in the future. Let's now turn to slide 9, grain production and offtake. Last fall, beginning in September 2022, we contracted with growers to plant WDH2 plant plots ranging from 30 to 160 acres. This contracting was done to set in motion steps in the value chain, from planting, to harvest, to grain delivery, to an offtake customer. The photos on this slide illustrate the steps of the value chain we have up and running in Western Canada. Our team contracted spring Camelina acres, which were planted in May. We expect harvest to be completed in the coming weeks.
In the third quarter, we are engaging with existing and new growers and seeing good interest in contracting winter acres for the 2023-2024 growing season. I'll now turn the call over to Kristi for an update on the development of next-generation seed genetics, particularly HT and stacked HT Camelina varieties.
Thanks, Oli, and good afternoon, everyone. Let's turn to slide 10. Growers need weed control to reduce competition for nutrients and increase the productivity of their crops. Our vision is to provide farmers with high-yielding, elite Camelina varieties, with herbicide-tolerance traits that are compatible with farmers' crop rotations. Incorporation of herbicide-tolerance traits in canola transformed it from a marginal crop to a major crop grown on millions of acres, and we believe Camelina may follow a similar path. Herbicide-tolerant lines of winter Camelina are particularly intriguing in that they could provide growers with a new crop rotation, a cover crop that increases grower revenue, protects soil health, and reduces nutrient runoff, all while producing a feedstock that can be used to produce a low-CI oil for biofuels.
Yield10 has also made progress in increasing the harvest value of Camelina, with programs to engineer traits for increased seed yield and oil content, improved protein meal value, and future higher-value seed products, such as omega-3 oils and PHAs. Yield10's technology development has resulted in a patent portfolio that includes 21 patent applications issued or pending. Let's now turn to slide 11. We have developed spring and winter lines of Camelina that have tolerance to an over-the-top spray of a broadleaf herbicide that is routinely used on major food crops. The chart on this slide shows progress towards achieving milestones to enable commercialization of our first herbicide-tolerant Camelina variety. We are making good progress on late-stage field trials and are waiting to hear back from USDA APHIS on regulatory filings for deregulated growth of the crop and from the Environmental Protection Agency to add Camelina to the herbicide label.
We have also initiated field work in 2023 to obtain seed samples for compositional analysis to support use of the seed meal for animal feed. We are also scaling up seed for commercial planting and are on track for a 2025 commercial launch of spring Camelina with tolerance to the broadleaf herbicide. Let's now turn to slide 12. We're also making good progress with stacked herbicide tolerance, which is the combined traits of tolerance to over-the-top applications of a broadleaf herbicide for weed control, combined with tolerance to Group 2 soil residues to achieve broader acreage. We recently reported encouraging data out of our ongoing spring 2023 field trial. We demonstrated robust stacked herbicide tolerance in multiple events. The photos show examples of events with stacked herbicide tolerance, with little or no growth of control plants without the trait.
We have initiated regulatory work with these lines and have filed a regulatory status review, or RSR, with USDA APHIS to deregulate the growth of camelina with the stacked trait. Going forward, we will harvest the field trials, measure seed yield and seed oil content, and choose the best lead and backup events to move forward in our pipeline. The lead event will be chosen based on robustness of the herbicide tolerance, as well as seed yield, seed oil content, and overall agronomics of the event. Seed from the lines will be scaled up in the winter of 2023/2024 contract season. We believe that the stacked herbicide tolerance lines will be a win-win for growers and Yield10, since the trait will allow robust weed control for farmers, increasing yield and revenue, and tolerance to previously used Group 2 herbicides, allowing broad expansion of acres available for planting camelina.
As I wrap up, I'd like to acknowledge the tremendous efforts of the Yield10 technology team that have enabled Yield10 to develop and employ cutting-edge technology to position camelina as a competitive crop for production of feedstock oils for biofuels. This technology package will also be used as the basis for the high-level production of Omega-3 oils in elite camelina backgrounds and the production of PHA bioplastics. In the coming months ahead, we look forward to further field testing our best commercial quality herbicide-tolerant camelina lines, as well as advancing the Omega-3 oil technology platform and securing regulatory clearances for our elite camelina products. I'll now hand the call back to Oli.
Thanks, Kristi. Now, let's turn to slide 13 for an update on omega-3. The omega-3 oils containing EPA and DHA fatty acids represent a very exciting market opportunity. Unlike the biofuel market, where we will sell grain to an offtake customer, this is a place where it will be possible to market the oil directly to end users. Our business strategy was set up to leverage the advanced Camelina varieties, including HT, an operating foundation established for biofuels, to build their business in omega-3 oils. We believe that Camelina will be an ideal platform crop for making these omega-3 oils. We are conducting our work in omega-3 oils in collaboration with Rothamsted Research in the UK. The team at Rothamsted developed the EPA8 Camelina line that produces up to 29% EPA in oil, depending on the background variety of Camelina used.
The EPA level in this oil is 2-3 times that of fish oil, we believe this can be developed as a preferred, higher-value source of purified EPA products, such as ethyl EPA, for the pharmaceutical and nutraceutical markets. They have also developed and extensively tested the DHA1 Camelina line, which contains 10% EPA and 10% DHA in the oil. Published aquafeed studies have shown that the DHA1 oil can serve as a drop-in replacement for fish oil. Clinical studies, also published, have shown this oil to be an effective alternative to fish oil in the human diet. Next-generation Camelina lines with further improvements to oil composition are also being progressed by Rothamsted, with funding support from Yield10. In July, we filed a request for regulatory status review, an RSR, with USDA-APHIS, as a key step in the regulatory path for omega-3 EPA Camelina.
We are currently developing a detailed plan for the commercial launch of this business. Let's now turn to slide 14, the market opportunity for omega-3. Both new challenges and drivers for new sources of the omega-3 fatty acids, EPA and DHA, both essential for human health and wellness, continue to emerge. Consumption of oily fish and salmon are the main sources of EPA and DHA fatty acids in the diet. There is tremendous pressure on the harvest of fish, such as anchovies, because it is proving to be unsustainable as natural fish stocks are declining. In fact, the anchovy harvest in Peru was canceled in 2023 due to low fish stocks.
Peruvian anchovy accounts for around 20% of global fish oil, so we expect this to have a significant additional impact on fish oil pricing near term, and in addition, result in increased third-party interest in alternative sources like the Camelina omega-3 platform. We know the regulatory hurdles are also being reduced. Norway, the largest producer of farmed salmon, until recently, had a moratorium on the use of GMO ingredients in aquafeed, but recently approved the use of a GMO omega-3 oil from Nuseed. They produce a DHA-containing oil in canola. Using the RSR process for regulatory approval by USDA-APHIS under the SECURE Rule, we see a clear path to production of omega-3 in the U.S. Chuck, over to you.
Thanks, Oli. Good afternoon, everyone. Let's turn to slide 15. We ended the second quarter of 2023 with $2.3 million in cash and cash equivalents. During Q2, we added $1 million to our cash position as a result of issuing a senior unsecured convertible note to Marathon Petroleum. This was in connection with the LOI we signed with them. We raised an additional $2.7 million in net proceeds from a registered direct offering in May. In addition, last week, we priced a $3.7 million public offering of units consisting of common shares and warrants. We expect that our cash on hand, including proceeds from these offerings, will support our operations into the fourth quarter of this year.
Our net operating cash used for operating activities was $3.2 million for the second quarter of 2023, as compared to $2.5 million for the second quarter of 2022. For the full year 2023, we continue to expect total net cash usage in the range of $12.5 million-$13 million to fund our operations, including payments to growers for our first camelina grain harvest. During our third and fourth quarters of 2023, we also expect to report our first camelina product revenues from camelina grain and sales to our biofuel offtake customers. Let's now review the second quarter and year-to-date 2023 operating results.
For the second quarter of 2023, the company reported a net loss of $3.7 million, or $0.64 per share, as compared to a net loss of $3.4 million, or $0.70 a share, for the second quarter of the previous year. As expected, due to the completion of our Department of Energy grant during our first quarter of 2023, we did not report grant revenue for the second quarter of this year. $0.1 million in grant revenue in the second quarter of last year. R&D expenses during the second quarter of 2023 and 2022 were consistent at $2 million, and G&A expenses were $1.7 million and $1.5 million, respectively.
For the six months ended June 30, 2023, the company reported a net loss of $7.5 million, or $1.39 per share, compared to a net loss of $6.8 million, or $0.38 per share, during the six months ended June 30, 2022. Year-to-date grant revenue from the completed DOE grant were $0.1 million and $0.3 million, respectively. Research and development expenses were $4.2 million and $3.8 million during the six months ended June 30, 2023, and June 30, 2022, respectively. General and administrative expenses were $3.4 million and $3.2 million for each of those periods. For more details on our financial results, please refer to our earnings release. Oli, back to you.
Thanks, Chuck. Let's now turn to slide 16 for upcoming milestones. Before getting into the upcoming milestones, I'd like to comment on the tremendous progress made by the Yield10 teams here in Massachusetts and in Canada. It's not easy for a small organization to make the transition from an R&D focus to commercial activities, with the added pressure of needing to demonstrate compelling capabilities on both to prospective strategic partners. No surprise then, that I'm extremely proud of the Yield10 team and what we have accomplished already this year. Biofuel partner agreements progressing with Marathon Petroleum and Mitsubishi. Additional biofuel partnership discussions ongoing with other oil majors and regional energy players. Winter Camelina, again, production and contracted in fall of 2022 and close to fully harvested, with some already delivered to an offtake partner. Spring Camelina production completed, with harvest coming shortly.
Finally, on the regulatory approval for omega-3 Camelina line EPA8, and achieving key milestones of developing HT, and very recently, stacked HT Camelina in field trials. Building on these accomplishments, we are working towards a number of key milestones in the second half of 2023 and into the first half of 2024, including first grain product revenue, a big milestone for us. Working with Marathon and Mitsubishi to finalize investment and offtake agreements. Continuing to progress partnerships with undisclosed third-party partner prospects interested in biofuel feedstocks. Contracting winter Camelina production with growers for planting this fall, as we continue to make progress introducing the benefits of growing Camelina as a winter crop and expand our grower network. Building seed inventory of current and HT, and stacked HT varieties for future grower contracts.
Developing and progressing the commercial launch of camelina omega-3 oils, and continuing business development outreach to prospective partners across their seed product areas with the goal of executing strategic industry collaborations. We'll continue to work on expanding our intellectual property portfolio. With that, I'd like to turn the call back over to Lynn for questions.
Thanks, Oli. Melissa, we're ready for questions.
Thank you. If you'd like to ask a question, please press star one on your telephone keypad. A confirmation tone will indicate your line is in the question queue. You may press star two if you'd like to remove your question from the queue. For participants using speaker equipment, it may be necessary to pick up your handset before pressing the star keys. Our first question comes from the line of Anthony Vendetti with Maxim Group. Please proceed with your question.
Thank you. Yeah, I just, you know, wanted to get an update first on the agreements. I know the LOI with Marathon Petroleum and then the MOUs with Mitsubishi and American Airlines. I'm just wondering where those are at in terms of moving to definitive agreements, and then I, I have some follow-up questions. Thanks.
Yeah, regarding, regarding, I mean, obviously with Marathon, I mean, there's a, there's a timeline associated with that LOI, which basically, I think, expires in. From the point of view of certain provisions in basically August 25th, I believe it is. So, you know, although it doesn't mean that, you know, conversation is over by then, that's when the exclusivity does run out. Obviously, we've been working very closely with Marathon over the last 3 months, basically, to structure the agreements. It'd be basically an agreement which will really define most of the parameters. I can't disclose those, as they're not public, but would basically involve 2 agreements. One would be an investment agreement, the second would be an offtake agreement.
Regarding Mitsubishi, we've made great progress with Mitsubishi and their partner, ENEOS, which is the major refiner, the largest refiner in Japan. They are also progressing pretty much along similar lines. We again, haven't disclosed the specifics of that, but certainly, we also anticipate two similar agreements, basically an investment agreement and a strategic offtake agreement for Camelina oil for the biofuel market. So, you know, with American Airlines, that's really more of a collaboration. American as an airline, and obviously, when you look at the potential demand for SAF, you know, 3 billion gallons by 2030, and 35 billion gallons in the US for SAF by 2050. Then you look internationally, the 13.6 billion gallons from Europe and another 6 billion gallons from Japan.
Certainly, the airlines, who are really going to be pretty much dependent on SAF as a major source of reducing their carbon emissions, you know, SAF is obviously very strategic to them. So we continue to work with American Airlines as they help to support us, to help develop this value chain. You know, essentially, American Airlines is not going to be an offtake partner or a refiner. Essentially, they have a strategic interest in seeing this industry expanded, particularly the availability of low CI non-food feedstocks.
Okay, yeah, no, that's, that's helpful, Oli. Just to clarify on Marathon Petroleum, they, they have until August 25th, so 11 days from now, to make a decision or the exclusivity with them lapses, and you could strike a deal with another company if you so desire. Is that correct?
Well, let's be clear. I'm not gonna get into that specifically. What I can say is that we are working very closely with Marathon to bring this to a positive conclusion. Within the constraints of the Marathon agreement, we are in discussions with other parties, including Mitsubishi and some others, in areas that are not constrained by, by that agreement. I'm not gonna provide any specifics on that.
Okay, that makes sense. Okay. You did say, though, by the end of this year, you'll be producing revenues from the Camelina grain.
That's, that's correct.
Correct? Correct. Okay. Can you talk about the where you're at with the commercialization process for the herbicide-tolerant Camelina and the interest from the farmers?
You know, I think I can talk to you about the interest from the farmers, very specifically, and then, obviously, on the technology front. You know, you, you've heard from Kristi's excellent presentation that... you know, great job by that team, obviously, not only progressing the, the, the herbicide tolerance for weed control, but the very rapid development and demonstration and field trials of the stacked herbicide technology. And we can talk to the, the merits of both of those. Fundamentally, what we hear from growers is, "We're very interested in this crop. We see tremendous potential for it. What are you gonna do about weed control?
You know, we need to have weed control in order to seamlessly integrate this into our crop rotations, so that we can grow this without, you know, having any negative impact on the other crops we grow. That's, that's number one. Number two is, in all of these areas, particularly in the Pacific Northwest, all the way down through Kansas, down into Texas, you know, they use a lot of these Group 2 herbicides. The industry has this thing called a, a plant-back date. If you've used a Group 2 herbicide on, on, you know, various plots of your land or sections of your land, as they call it, you know, basically, there are restrictions on what you can plant on that land, you know, soon thereafter.
Sometimes for Group 2s, those can be, you know, a few months to as much as a year or even longer. That has a major... What it does is it reduces the number of acres each grower has available, potentially planting winter Camelina or even spring Camelina, for that matter. By having the stacked HT, it has tolerance to those residues in addition to the weed control. We're just, you know, quite frankly, we're making this idiot-proof for the grower to adopt these on a very large scale. Certainly, the conversations we've had with growers indicate that they're very excited about that.
Okay, great. Thank you so much. I'll hop back in the queue. Appreciate it.
Thank you. Ladies and gentlemen, as a reminder, if you'd like to join the question queue, please press star one on your telephone keypad. We'll pause a moment to allow for any other questions. At this time, I'm seeing no other questions. Ms. Brum, I'll turn the floor back to you for any final comments.
Great. Thanks, Melissa, and I'll turn the call back to Oli.
I'd like to thank, you know, personally thank all of you for joining us on the call tonight, and especially our shareholders for your continued support. I want to thank everyone at Yield10 for contributions that are keeping us on track, future commercial and product development goals. Have a nice evening, everyone.
Thank you. This concludes today's conference call. You may disconnect your lines at this time. Thank you for your participation.