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Cowen Health Care Conference

Mar 8, 2023

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Everyone for joining us for the Alnylam Pharmaceuticals fireside chat, at the 43rd annual TD Cowen Conference. I'm covering analyst Ritu Baral. With us today from Alnylam, we have Pushkal Garg, CMO, as well as Rena Denoncourt, VP and TTR Program Lead at Alnylam. Welcome, Rena and Pushkal.

Rena Denoncourt
VP and TTR Program Lead, Alnylam Pharmaceuticals

Pleasure.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Good to have you back.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

Thank you.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

All right. Let's start with your SNDA. You know where I'm going with this. Have you had any more recent communication with the FDA about the SNDA for ATTR-CM? Have you been notified of any emerging review issues? You mentioned that at one point you had been told there were none.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

Yeah. First of all, good to see you, Ritu, and thanks to you and TD Cowen for inviting us here today. Really excited to be participating in the conference. Look, in terms of our ONPATTRO or patisiran review of the SNDA, you know, we announced, as we did on February 17th, that we'd gotten a letter saying that the file had been accepted, that the FDA was planning for an advisory committee, and that there were no review issues identified to date. You know, there's always, in the context of a review, some back and forth that happens in terms of data clarifications, et cetera. In the context of the AdCom, we don't have any more information at this point.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Okay. If FDA identifies a review issue as part of the SNDA, are they mandated by the statute, by PDUFA, to tell you?

Pushkal Garg
CMO, Alnylam Pharmaceuticals

Look, I think the, you know, I think the FDA is going to be doing their review and going through all the data that's been provided to them, and it's their prerogative what they want to raise to us and when. Certainly we're, you know, always going to be facilitating the review. When issues are raised to us, we'll certainly address them.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Okay. you know, I'm still trying to wrap my head around the fact that they know they want an AdCom, but they don't know of a review issue. What are the topics then that you're then preparing around? Because usually most developers will just go straight to the review issues. What topics are you prepared around? How do you even start preparation if you don't have review issues to serve as the core of the conversation?

Pushkal Garg
CMO, Alnylam Pharmaceuticals

Yeah. I think maybe a couple points there. First of all, I think it's important to note that, you know, as part of FDA's armamentarium of things that they can do in terms of reviewing an application is, it's their prerogative to convene an advisory committee of independent experts to give them advice. That's part of the process. Again, it's something that we'll support. We don't have specific insights at this point. We may learn in due course of issues and topics that the FDA wants to raise to the panel. You know, it's possible, while I don't have any specific information, that this may fall in the realm of efficacy. You know, you'll recall that the FDA issued guidance in 2019 on heart failure.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Yes.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

As part of that was where they announced that both, you know, functional, measures like the six minute walk test, quality of life measures like the KCCQ, in the absence of outcomes could be registrable endpoints for heart failure. To our knowledge, patisiran would be the first drug, reviewed under that, under that guidance.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

That guidance did not have any specified threshold for effect size for either of those two?

Pushkal Garg
CMO, Alnylam Pharmaceuticals

Not to our knowledge. We're waiting to see what the FDA brings forward, and they will, you know, raise in due course the, what the topics that they wanna cover. As far as we in our preparation, look, we're here to really facilitate the agency's review and the questions that the advisory committee panelists will wanna ask. We feel very good about the study that we've designed, conducted, executed, and the data that we have. We'll be preparing across all fronts. That's our responsibility, is to be able to speak to all aspects of the study and the results. You know, design, conduct, safety, efficacy, statistics, clinical pharmacology, whatever it is, we'll be prepared to speak to that.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

This would potentially be the first heart failure-related approval on six minute walk in Kansas City, KCCQ, correct?

Pushkal Garg
CMO, Alnylam Pharmaceuticals

To our knowledge. It would in the absence of the outcomes data.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Which would then present precedent for future applications for future developers.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

Potentially.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Potentially.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

Yeah. I don't speculate. Yeah.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Are you gonna be submitting 120-day safety data?

Pushkal Garg
CMO, Alnylam Pharmaceuticals

We will. We will be. You know, as part of our obligations is to provide a safety update to the agency, and so we'll be doing that, as you noted, 120 days roughly after the mark, we kinda do that update.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Well, aside from that, are there any, again, anything in the statute, anything in the PDUFA statutes that specifically mandate or prescribe additional communication points in an SNDA? I'm a little triggered by this. I've been burned before trying an SNDA, so I'm hoping you're telling me that you can tell me that there's a point of communication that you are expecting that's in PDUFA.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

Yeah. you know, from our understanding, you know, in the context of an SNDA, there's not some of the typical things that you might have for original NDA in terms of, for example, mid-cycle review meetings, et cetera. We do expect that in the context of planning for an advisory committee, that the guidance would indicate that the FDA will let us know more about the topics for that advisory committee roughly two months before scheduling the meeting, before the scheduled date of the meeting.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Got it.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

We'll look forward to hearing more from the agency. Again, it's their prerogative as they conduct the review. They'll keep us posted, and we'll certainly be responsive to any requests and needs that they have.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Understood. How are you gearing up for the potential launch? I mean, you've indicated that ONPATTRO for these patients is going to be for the most, not your words, mine, but the most desperate patients with the biggest unmet need, which is a seemingly smaller population. Are you going to be expanding call points, hiring additional reps? How are you thinking about that?

Rena Denoncourt
VP and TTR Program Lead, Alnylam Pharmaceuticals

Let's talk about the patient population in a bit.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Yeah.

Rena Denoncourt
VP and TTR Program Lead, Alnylam Pharmaceuticals

To your specific question about the call points, there are a number of new treating physicians who would be potentially treating wild-type ATTR amyloidosis.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Mm-hmm.

Rena Denoncourt
VP and TTR Program Lead, Alnylam Pharmaceuticals

-or the patients with the cardiomyopathy of hATTR amyloidosis, in addition to the physicians that we see today. I would say we would be planning to expand both our commercial and medical teams to support a potential launch. That's included in our OpEx guidance that has been provided.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Mm-hmm.

Rena Denoncourt
VP and TTR Program Lead, Alnylam Pharmaceuticals

Further granularity to that, it's a competitive space.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Sure.

Rena Denoncourt
VP and TTR Program Lead, Alnylam Pharmaceuticals

We aren't going to get into, you know, much more detail there. I think importantly, we have a well-established infrastructure of our commercial teams, with the field-facing teams for physicians, for payers, and we'll be leveraging that. Really not building this from scratch.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Mm-hmm.

Rena Denoncourt
VP and TTR Program Lead, Alnylam Pharmaceuticals

Rather, taking the knowledge and the relationships that we have established and being able to position ourselves well for that.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Well, is a cardiology call point, is a cardiology rep, a materially different higher than a rare neuro rep?

Rena Denoncourt
VP and TTR Program Lead, Alnylam Pharmaceuticals

I think we're looking for general traits in a rep that are able to take a specialty call point.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Mm-hmm.

Rena Denoncourt
VP and TTR Program Lead, Alnylam Pharmaceuticals

It's really taking new HCPs and new centers of excellence.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Mm-hmm.

Rena Denoncourt
VP and TTR Program Lead, Alnylam Pharmaceuticals

That many of them have already been seeing patients, with hATTR amyloidosis with polyneuropathy at these centers of excellence. They see all the different forms.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

I see.

Rena Denoncourt
VP and TTR Program Lead, Alnylam Pharmaceuticals

of ATTR amyloidosis.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Okay. Yeah.

Rena Denoncourt
VP and TTR Program Lead, Alnylam Pharmaceuticals

as we expand.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Mm-hmm.

Rena Denoncourt
VP and TTR Program Lead, Alnylam Pharmaceuticals

Kind of potentially expand the label, there'll be many pieces of the existing footprint that we can leverage.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Got it.

Rena Denoncourt
VP and TTR Program Lead, Alnylam Pharmaceuticals

They either have experience, these treating physicians may have experience with ONPATTRO for the treatment of the polyneuropathy manifestation of disease.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Mm-hmm.

Rena Denoncourt
VP and TTR Program Lead, Alnylam Pharmaceuticals

They have experience already seeing these types of patients because they're the mixed patient population that is going to these potential treating physicians already. There's a lot to leverage there.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Understood. I think I swore I had that. Any changes to the hub around this, and what do you think the profile of the ideal ONPATTRO ATTR cardiomyopathy patient is?

Rena Denoncourt
VP and TTR Program Lead, Alnylam Pharmaceuticals

Sure. Nothing materially changes in the hub.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Mm-hmm.

Rena Denoncourt
VP and TTR Program Lead, Alnylam Pharmaceuticals

It's kind of consistent with our existing approach of making sure that we work diligently to make sure that patients who are prescribed our treatments can get access to them.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Mm-hmm.

Rena Denoncourt
VP and TTR Program Lead, Alnylam Pharmaceuticals

Get the support that they need for treatment. When we're thinking about-

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Sure.

Rena Denoncourt
VP and TTR Program Lead, Alnylam Pharmaceuticals

The potential indication in ATTR amyloidosis with cardiomyopathy, the existing landscape is that these patients are experiencing disease progression or inadequate treatment.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Yeah.

Rena Denoncourt
VP and TTR Program Lead, Alnylam Pharmaceuticals

There remains to be a significant unmet medical need in this population. You have patients who are getting access to the existing therapy and having an inadequate response, or you have patients who can't even get access.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Mm-hmm.

Rena Denoncourt
VP and TTR Program Lead, Alnylam Pharmaceuticals

to therapy.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Sure.

Rena Denoncourt
VP and TTR Program Lead, Alnylam Pharmaceuticals

Those are some examples of patient populations that might have the potential to benefit from ONPATTRO, should we get the approval in that indication.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

What do you expect the approximate... I think this is independent of pricing, but with the price, Part B copay, for these patients?

Rena Denoncourt
VP and TTR Program Lead, Alnylam Pharmaceuticals

Right. What the important piece is there is that the out-of-pocket copay and the.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Mm-hmm.

Rena Denoncourt
VP and TTR Program Lead, Alnylam Pharmaceuticals

The payer dynamics are not cost-prohibitive for patients.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Mm-hmm.

Rena Denoncourt
VP and TTR Program Lead, Alnylam Pharmaceuticals

to get access to it. Further details along that, it's still a little too early to discuss. What we've seen in the polyneuropathy space, of course, is good low barriers to having patients get access to ONPATTRO for polyneuropathy or AMVUTTRA for polyneuropathy because of streamlined access processes.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

The Part B copays do tend to be lower than the Part D copays, correct?

Rena Denoncourt
VP and TTR Program Lead, Alnylam Pharmaceuticals

They do.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

And-

Rena Denoncourt
VP and TTR Program Lead, Alnylam Pharmaceuticals

In general.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

In general. Would this really expand a real possibility for combination with the stabilizer, upon potential approval?

Rena Denoncourt
VP and TTR Program Lead, Alnylam Pharmaceuticals

Again, it's early.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Right.

Rena Denoncourt
VP and TTR Program Lead, Alnylam Pharmaceuticals

Without having a label and without, you know.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Hypothetically.

Rena Denoncourt
VP and TTR Program Lead, Alnylam Pharmaceuticals

Approval, to be determined, right.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Mm-hmm.

Rena Denoncourt
VP and TTR Program Lead, Alnylam Pharmaceuticals

We want the physician to make the decision based on the data.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Sure.

Rena Denoncourt
VP and TTR Program Lead, Alnylam Pharmaceuticals

based on what the individual patient needs would be. those are.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Nothing in the statute says no, you couldn't.

Rena Denoncourt
VP and TTR Program Lead, Alnylam Pharmaceuticals

A statute.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

just in, like Medicaid, Medicare, there's no...

Rena Denoncourt
VP and TTR Program Lead, Alnylam Pharmaceuticals

That would be, you know, based on the individual plans and the expectations for the individual patient.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Yeah.

Rena Denoncourt
VP and TTR Program Lead, Alnylam Pharmaceuticals

It's theoretically possible.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Mm-hmm.

Rena Denoncourt
VP and TTR Program Lead, Alnylam Pharmaceuticals

Again, label to be determined and things like that.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Of course. You're not pursuing the biannual treatment regimen anymore?

Rena Denoncourt
VP and TTR Program Lead, Alnylam Pharmaceuticals

Right.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Has that had any impact to your internal models about how much ONPATTRO could sell for cardiomyopathy?

Rena Denoncourt
VP and TTR Program Lead, Alnylam Pharmaceuticals

The biannual regimen is for vutrisiran.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Oh, yeah. I'm sorry.

Rena Denoncourt
VP and TTR Program Lead, Alnylam Pharmaceuticals

Yeah.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

On, for, AMVUTTRA could sell for cardiomyopathy.

Rena Denoncourt
VP and TTR Program Lead, Alnylam Pharmaceuticals

Right. For AMVUTTRA. Let me, maybe review some of the rationale for

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Mm-hmm.

Rena Denoncourt
VP and TTR Program Lead, Alnylam Pharmaceuticals

Strategic decision to not further pursue the biannual dosing regimen at this time, and it's really three different reasons.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Mm-hmm.

Rena Denoncourt
VP and TTR Program Lead, Alnylam Pharmaceuticals

-that have come together to lead us to this decision. The first is that the 25 mg quarterly regimen of AMVUTTRA has had a very, a very successful early launch. The first six months of that have been exceeded our expectations. We're really, really pleased with what we've seen there, and it's been essentially a game changer for this space.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Mm-hmm.

Rena Denoncourt
VP and TTR Program Lead, Alnylam Pharmaceuticals

You have the efficacy, the safety, and the quarterly regimen that uptake from physicians and patients has been, we're very pleased to-

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Mm-hmm.

Rena Denoncourt
VP and TTR Program Lead, Alnylam Pharmaceuticals

to see the favorable results there. When you look at the biannual dosing data, the 50 mg biannual regimen, the study was the HELIOS-A randomized treatment extension. We did confirm that it was the mean TTR knockdown was non-inferior to the 25 mg regimen.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

At nine months.

Rena Denoncourt
VP and TTR Program Lead, Alnylam Pharmaceuticals

Essentially for two doses. Yes.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Mm-hmm.

Rena Denoncourt
VP and TTR Program Lead, Alnylam Pharmaceuticals

It achieved its intended endpoint on the non-inferiority and the safety profile. What we did see was some return of the TTR reduction at the end of the biannual regimen, so towards the end of the six months.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Yes.

Rena Denoncourt
VP and TTR Program Lead, Alnylam Pharmaceuticals

That's not aligned with the optimal target product profile that we would like to bring forward.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

You want coverage. You want good coverage.

Rena Denoncourt
VP and TTR Program Lead, Alnylam Pharmaceuticals

You want robust and sustained TTR knockdown. The third element that's really relevant to this decision is the TTRsc04 molecule.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Yeah.

Rena Denoncourt
VP and TTR Program Lead, Alnylam Pharmaceuticals

that's right, behind and just started dosing in the phase I study. Having that as an option that we can now focus on to provide another option of bringing innovation to patients with the potential for the robust and sustained efficacy and the infrequent dosing that molecule has the potential to provide, kind of led us to say, "Let's, let's streamline this, franchise development and focus on sc04 for that next molecule in the, in the line.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Were there potential IRA considerations into that strategy as well?

Rena Denoncourt
VP and TTR Program Lead, Alnylam Pharmaceuticals

No. No, that was not key to the decision.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

On HELIOS-B, can you review for us what you've said on the powering of the study, TAF baseline TAF use and allowances and observations for TAF drop-ins?

Pushkal Garg
CMO, Alnylam Pharmaceuticals

Yeah. Maybe a couple points there, right? HELIOS-B is our outcomes-based study for AMVUTTRA or vutrisiran in ATTR-CM. I think first and foremost, we're really encouraged because the APOLLO-B data that we talked about at the beginning of this discussion.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Mm-hmm.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

really portends well, we believe, for HELIOS-B, right? We validated the therapeutic hypothesis that silencing with an RNAi therapeutic can actually have beneficial effects across a whole range of endpoints in this disease. Clinical biomarkers, echocardiographic, technetium, and from a safety perspective. That, first of all, really helps us a lot in terms of thinking about all of those are important prognostic factors that relate to outcomes, right? HELIOS-B is, you know, roughly twice as large, and instead of being a 12-month study, goes out to 36 months, 30-36 months. It's considerably larger and longer. We feel very good about the design and conduct of the study. As you know, we tend to be pretty conservative in our powering assumptions, and we feel really good about that.

We have factored in that up to 50%. What's that?

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

You won't tell us what they are.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

We have factored in that, you know, allowance of up to 50% was our operational target.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Mm-hmm.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

in terms of percent of patients on baseline tafamidis. We've come in south of that. you know, the last we've spoke about, when we talked about taf drop-in, those rates have been low, and we've been comfortable with that as well. Overall, we feel really good about the design and the conduct of the study, and we're looking forward to seeing the results in early 2024.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Are you looking at the blinded event rate?

Pushkal Garg
CMO, Alnylam Pharmaceuticals

We have teams, you know, in any clinical trial that we're doing.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Mm-hmm.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

We have teams looking, at the data, overall, primarily for study integrity purposes.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Right.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

-to make sure that the study's being conducted properly at the sites, that there's outlier data points, those are addressed, et cetera. As part of that, they are looking at blind data and, you know, we're very comfortable with what they're seeing. We feel good, again, about the conduct of the study. It's also being reviewed by a data monitoring committee.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Mm-hmm.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

They also continue to, you know. We feel good about the fact that their reviews have been uneventful. Everything is moving forward.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

In the event that the event rate was coming in way too low, something would be triggered internally at Alnylam. There would be an awareness of that underpowering, potential underpowering.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

Yeah.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Hypothetically.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

I think we are really committed to bringing forward this medicine to patients with this disease. It's obviously there's a huge unmet need, as Rena referred to. We wanna bring forward this medicine, and we're gonna do everything that we can to optimize our clinical trial to deliver that. Again, we feel good about what we've designed and what's being executed here.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

You said, I think it was you who said on the earnings call that you have no current plans to change the analysis to an event-driven plan. This is something you and I discussed in January. Could you? Is it impossible?

Pushkal Garg
CMO, Alnylam Pharmaceuticals

I mean, it is, Ritu, so speculative.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Yeah.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

I think what I would say is we think about all the different possibilities in terms of optimizing the design and conduct of the trial.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Mm-hmm.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

we feel good about where we're at.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Okay. The next catalyst people are focused on ALN-APP.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

Yeah.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

You set the bar at 50% knockdown. Just to clarify for folks, this is the protein itself in the CSF, correct?

Pushkal Garg
CMO, Alnylam Pharmaceuticals

Not exactly. What we're looking at, maybe just as a, you know, zooming out for a moment.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Mm-hmm.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

This is our first foray with an RNAi therapeutic into the CNS.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Mm-hmm.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

It's a really exciting opportunity to take all the learnings that we've gotten from liver-directed RNAi therapeutics now into a new tissue compartment.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Mm-hmm.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

where there's a whole bunch of diseases that I don't have to educate anyone in this room about that have tremendous unmet need and with their genetic underpinnings that we can potentially address. This becomes a very important medicine in that regard, and ALN-APP itself is targeting and being addressed for patients with potentially Alzheimer's disease and another disease called cerebral amyloid angiopathy, with tremendous unmet need. The data that we're the phase I study that's going on right now, that we'll be planning to report data out on in the first half of this year and in the second quarter is really from the is an interim look at data from the single ascending dose portion of the study. We'll be looking for, you know, to show safety and tolerability.

This is the first time delivering a drug into the CSF space, an RNA therapeutic, and also then knockdown. To your point.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Knockdown.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

To your point around that, you know, we're not measuring APP directly, but we're measuring downstream cleavage products of that.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Mm-hmm.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

which is soluble APP alpha and soluble APP beta. These are very good you know, these are proximal indicators of what level of knockdown that we're seeing.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Got it. It's the soluble APP alpha, soluble beta that you are expecting to hit that 50% threshold.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

Yeah. What I would say is, just to be clear, this is an interim look at data emerging from the study. This doesn't necessarily mean it's going to be the final results of all the dose escalations, right?

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Okay.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

When we talk about 50%, that's really a crude threshold from the context of when we've been asked, you know, what is the level of targeting that we'd like to see.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Mm-hmm.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

Where we might expect to see therapeutic benefits in longer-term clinical trials, right?

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Yeah.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

That comes from genetic data, where we think that if we can get to about 50%, you know, that would be a good hypothesis to be exploring in longer-term trials and larger numbers of patients with Alzheimer's disease or CAA. Right now, we're in the dose escalation phase.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Mm-hmm.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

We're really just looking for safety, tolerability, target engagement, dose-related knockdown, durability, factors like that.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Is there preclinical support for that 50% or genetic support for that 50%?

Pushkal Garg
CMO, Alnylam Pharmaceuticals

There's some genetic support for that 50%.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Mm-hmm.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

I will say that, you know, models here, preclinical data are limited, right?

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Mm-hmm.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

We have a strong therapeutic hypothesis that, you know, the antibodies have actually validated that, you know, the A Beta hypothesis that lowering amyloid can be beneficial. Certainly, there's a tremendous amount of residual unmet need.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Right.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

I think all of us would believe, you know, want to see better therapies for patients with Alzheimer's disease. We think that by this mechanism, by going upstream, we can actually turn off the tap here in terms of amyloid production. Not only of full-length amyloid, but various oligomers and fragments that are developed, and not just extracellularly in the parenchyma of the brain...

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Mm-hmm.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

Actually intracellularly as well. We think we have the potential to have a more upsized benefit in this disease. That remains to be seen, and we'll do the clinical trials to do that. Again, in accordance with that, we think the genetic data would suggest that getting to about 50% knockdown.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Mm-hmm.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

should put us in the range to explore therapeutic efficacy.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Are there any regional or structural considerations when we think about the alpha and beta, APP, soluble APP alpha beta reductions? I mean, it's a disease of cognition.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

Mm-hmm.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Of course, that tissue is easy to access. It's just the gray matter, essentially on the outside. Basically, if that occurs, if it's only knocked down on those tissues, sort of on the outside of the brain rather than the deep brain tissue.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

Mm-hmm.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Would that have any implications to potential downstream clinical effects?

Pushkal Garg
CMO, Alnylam Pharmaceuticals

No, I think, you know, we do know that in Alzheimer's patients, certainly that there's amyloid that's deposited in multiple regions of the brain. You know, certainly, there's a lot of cortical involvement potentially.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Mm-hmm.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

particularly in more advanced disease. We're, you know, we're going to be measuring the APP alpha and beta in the, in the cerebrospinal fluid.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Yeah.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

which is really going to be coming from the total mass of the brain.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Mm-hmm. Got it. Is there any way to figure out if you're seeing knockdown in the deep brain structures?

Pushkal Garg
CMO, Alnylam Pharmaceuticals

There's no, you know, we're not directly measuring that.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Mm-hmm.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

I mean, certainly what we've seen from non-human primate studies, however, really tells us that this mechanism, we are able to actually get access deeper brain structures and a variety of cell types. We've shown data, now, looking at that-

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Mm-hmm.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

You can see, good difference.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

What cell types?

Pushkal Garg
CMO, Alnylam Pharmaceuticals

Across, you know, neurons, astrocytes, glial cells, and into deeper structures in terms of striatum, thalamus, et cetera.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Okay.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

A variety of cell types and tissues.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

That's non-human primate brain?

Pushkal Garg
CMO, Alnylam Pharmaceuticals

That's non-human primate data.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Got it.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

Yeah.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Okay. Any other biomarkers you're gonna be releasing with the top-line data? And if so, what do they mean and what kind of movement?

Pushkal Garg
CMO, Alnylam Pharmaceuticals

Yeah. I think the initial tranche of data will be focused primarily, as I said, on safety tolerability in soluble APP alpha and beta. In the context of the study, we are measuring a number of other parameters. There's other CSF and plasma biomarkers that relate to target engagement, Aβ40 and 42, markers of inflammation, et cetera. We will also be doing neuroimaging through a variety of modalities, including volumetric MRI, et cetera.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Mm-hmm.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

Those are gonna come in due course.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Not-

Pushkal Garg
CMO, Alnylam Pharmaceuticals

Those will not be the part of the initial tranche of data.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Got it. All right. Let's move to zilebesiran in the upcoming KARDIA-1 and KARDIA-2 readouts.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

Yeah.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

later this year. What should people's expectations for the milligrams of mercury improvement be for? Let's start with KARDIA-1 monotherapy on its own. No background treatment. Remind us what you saw in the two, the phase I/II on monotherapy.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

Mm-hmm.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

any reason to expect anything different in KARDIA-1?

Pushkal Garg
CMO, Alnylam Pharmaceuticals

zilebesiran, a very exciting program that can really fundamentally be transformational potentially for the treatment of hypertension and reducing cardiovascular disease. The ability to sort of tonically control blood pressure for prolonged periods of time, it's really very, very differentiated from anything that's come before and sort of addresses the fundamental problems we've had with blood pressure control. In the phase I study, what we saw was we took single doses up to 800mg , and we saw dose-related decreases in angiotensinogen and in blood pressure. We saw systolic blood pressure reductions at the highest dose of up to 20mm of mercury and lasting for up to six months.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Mm-hmm.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

Really exciting profile and good safety and tolerability with that. The KARDIA-1 study is really trying to now expand on that experience. We're looking at a range of doses from 150mg- 600mg .

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Mm-hmm.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

regimens of every three months and every six months. Right. The goal here is to actually come up with the dose and regimen that will be used in pivotal trials.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Mm-hmm.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

Any blood pressure reduction of 5mm diastolic is considered clinically significant. A 5mm systolic blood pressure reduction roughly associates or correlates with a 10% reduction in cardiovascular morbidity and mortality. It's quite clinically significant. I think based on the data that we saw in the phase I, we'll expect, you know, maybe even see something higher than that.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Even higher? Okay.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

Than the five, yeah.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Than the five.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

Yeah.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

how should we think about KARDIA-2?

Pushkal Garg
CMO, Alnylam Pharmaceuticals

Yeah.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

wrote that wrong. It's the combo therapy.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

Yeah. Yeah, yeah.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

You know, how will real-world use across larger centers and maybe better background control or maybe less good background control...

Pushkal Garg
CMO, Alnylam Pharmaceuticals

Yeah.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

how should we be thinking about that magnitude of millimeter benefit?

Pushkal Garg
CMO, Alnylam Pharmaceuticals

Yeah. look, I think what we'll look to do there, I mean, both between KARDIA-1 and KARDIA-2, we're now expanding across them. We're gonna have almost 1,000 patients.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Mm-hmm.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

in, you know, on this drug.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Mm-hmm.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

I think that's gonna be really helpful to understand, and also patients with more cardiovascular risk factors or comorbidities.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Yeah.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

We'll be exploring closer to the populations that we'll be studying in pivotal trials. You know, we're targeting this drug ultimately for patients with uncontrolled hypertension who are at high cardiovascular risk. Many of these patients are gonna be where there's a high unmet need.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Mm-hmm.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

An urgency to treat those patients and get their blood pressure controlled. For example, patients have a stroke or an MI, they have uncontrolled hypertension. You know, if you don't bring that blood pressure down, they will be at higher risk for having a recurrent event.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Mm-hmm.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

These patients are also now taking multiple medicines and polypharmacy is quite common in hypertension management. KARDIA-2 will really start to develop the data set about how does zilebesiran combine with other commonly used classes of medicines.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Yeah.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

Diuretics, calcium channel blockers, and angiotensin receptor blockers. In terms of the magnitude of effect, you know, as you get on patients and you're using a therapy on top of one or two or other medicines, you often will expect to see that treatment effect may be somewhat lower.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Mm-hmm.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

There's also synergies that happen. We know that RAS blockers combined with a diuretic can often have, you know, enhanced efficacy. We're very excited to see what that data set looks like. I think another interesting aspect of the data set will be how does it combine with an angiotensin receptor blocker.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Yeah.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

We had some experience, both we've got pre-clinical data, but also phase I experience that says in, you know, in contrast to what may have been seen previously when you'd use two RAS blockers together, where there's really not additive efficacy. Again, in a very small cohort of patients, of about 12 patients, we saw about a 6.5mm increase or additive benefit of giving of combining the two agents. That's encouraging with no safety concerns emerging there. This will be a much larger experience of looking at that combination.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Mm-hmm.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

It'll be quite interesting to see if there's additive efficacy and lack of safety concerns.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Just very quickly, you know, as we think about everything happening, KARDIA-1 and KARDIA-2 coming to fruition, APP at potential phase two start, and the potential ATTR-CM launch, how are you thinking about OpEx increases over the next three years as you know, as you reiterate a break even, I think, in 2025?

Pushkal Garg
CMO, Alnylam Pharmaceuticals

Mm-hmm.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Which was the important. Jeff finally put a year on it for so many years. Mid-term break even. 2025, break even. Is there gonna be a significant... Is he giving you a lot of money for that?

Pushkal Garg
CMO, Alnylam Pharmaceuticals

Um-

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Money to anybody.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

Jeff's been very kind to us, in development. look, I think we are very committed to our P to the fifth by 2025 goals, which includes profitability within the period. I think that really comes from two aspects. One is growing the top line.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Mm-hmm.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

We've committed to, you know, an average of a 40% CAGR over the period. That's gonna be driven by all, you know, the existing products as well as what launches in hopefully cardiomyopathy. Then tempered and then balanced against that will be, continued investments in R&D and in the pipeline, and in OpEx overall. But maybe as an illustrative point, you know, in 2021, OpEx grew by about 16%. Last year was about 14%. Then the guidance, that Jeff just provided at our last earnings call, you know, we're forecasting the midpoint of the range of OpEx increase of about 13%.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Mm-hmm.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

We really are trying to temper that growth as we get to be a larger and more mature company because we are very committed to sort of meeting that profitability goal. That said, we have a very rich set of opportunities ahead of us. We have a very productive R&D engine, it's important that we continue to invest both in bringing more products forward, but also enhancing in our ability to bring medicines to patients in terms of investing in our AMVUTTRA field force, et cetera. We'll be working on all those things, but certainly you'll see a tempering.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Mm-hmm.

Pushkal Garg
CMO, Alnylam Pharmaceuticals

-of that and discipline around the OpEx, and the continued efforts on growing the top line. We will be investing in the company.

Ritu Baral
Managing Director and Senior Biotechnology Analyst, TD Cowen

Great. Well, thank you, Pushkal. Thanks, Rena, for joining us and for the insight. Appreciate it. Thank you for joining us.

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