Quarter Earnings Call. At this time, all participants are in listen-only mode. Following the formal remarks, we will open the call up for your questions. Please be advised that the call is being recorded. At this time, I'd like to turn the call over to Lavina Talukdar, Head of Investor Relations at Moderna. Please proceed.
Thank you, Tamara. Good morning, everyone. Thank you for joining us on today's call to discuss Moderna's Q1 2021 Financial Results and Business Updates. You can access the press release issued this morning, as well as the slides that we'll be reviewing by going to the investor section of our website. On today's call are Stéphane Bancel, our Chief Executive Officer, David Meline, our Chief Financial Officer, Stephen Hoge, our President, Tal Zaks, our Chief Medical Officer, Corinne Le Goff, our Chief Commercial Officer, and Juan Andrés, our Chief Technical and Operations Officer. Before we begin, please note that this conference call will include forward-looking statements made pursuant to the safe harbor provision of the Private Securities Litigation Reform Act of 1995.
Please see slide two of the accompanying presentation and our SEC filings for important risk factors that could cause our actual performance and results to differ materially from those expressed or implied in these forward-looking statements. We undertake no obligation to update or revise the information provided on this call as a result of new information or future results or developments. On slide three, please see the important indication and safety information for our COVID-19 vaccine, which has been authorized for emergency use in the U.S. and many other countries around the world. I will now turn the call over to Stéphane.
Thank you, Lavina. Good morning or good afternoon, everyone. Thank you for taking the time to join our Q1 2021 Conference Call. We'll start by a quick business review of the quarter before Corinne walks you through a commercial update. David will walk you through the key financials. Stephen will provide a clinical update, especially new human data about two of our COVID-19 booster candidates, mRNA-1273, the currently authorized vaccine, and mRNA-1273.351, the variant-specific booster to B.1.351 first identified in South Africa. I will come back to close. The Moderna COVID-19 vaccine is now available and protecting people in 37 countries around the world. With the WHO authorization last Friday night, the number of countries where our vaccine will be available will go up significantly.
In the Q1 alone, 102 million doses have been shipped, and many tens of millions of people have been fully vaccinated or received their first dose. 12 months ago, in Q1 2020, Moderna had never run a phase III clinical study, never gotten a product authorized by a regulator, and never made 100 million doses in a single quarter. Not even 10 million, not even one million doses. I am very proud of what the Moderna team has achieved. Most importantly, I am very thankful for their impact on the world and the incredible personal sacrifices that our team has made to help protect fellow human beings around the world. This is very humbling, I'm fortunate to lead Moderna in this moment.
I'm also thankful for Moderna scientists, engineers, doctors, and team members who have worked relentlessly over the last 10 years now to be ready for when the virus emerged in late 2019. We invented the technology to produce safe, well-tolerated mRNA vaccines, which made it possible for us to chase this virus. The company achieved revenues of $1.9 billion in Q1 2021, of which $1.7 billion were COVID-19 vaccine product sales. The net income over the period was $1.2 billion. This marks the company's first GAAP profitable quarter in its history after nine years of operating losses. At the end of 2021, we had cash and cash investments of $8.2 billion. David will give you more details in a few minutes. We increased our 2021 supply forecast once again.
We now believe that we should be able to supply 800 million doses in 2021, and we're still aiming for 1 billion doses for the year. The total advance purchase agreements signed for delivery in 2021 have been increased to $19.2 billion. We are happy to report this morning an interim update to our TeenCOVE study. The initial interim analysis of a phase II/III TeenCOVE study of mRNA-1273 showed vaccine efficacy against COVID-19 of 96%, and mRNA-1273 was generally well-tolerated with no serious safety concern identified to date. We're also on track to start this month the filing of our rolling BLA to the FDA for COVID-19 vaccine mRNA-1273. Outside of COVID, we added another first as a company, and that is the dosing of our first patient with an mRNA therapeutics candidate against a rare genetic disease, propionic acidemia, a genetic deficiency in the liver with our candidate mRNA-3927.
One of the things that I'm the most excited about is where we are going. The Q1 results highlighted above are the consequence of last year's work and decisions we made. As I look to where Moderna is going, I get very excited by the level of our increased investment across the board, using our strong balance sheet to invest to scale Moderna. Just two numbers for some color. In Q1 2021, our R&D investments were approximately four times higher than R&D investments in Q1 of last year. Not 4%, not 40%, four times higher. For all of you who have known us for many years, Moderna has been built as a digital enterprise since the early days. We now have the opportunity to do much more and to build new functions like clinical trial operations, pharmacovigilance, commercial, digitally from the get-go.
Looking at the next 5 to 10 years, we're investing intensely in digital, automation, and AI. Our plan for 2021 is to invest three times more in digital than in fiscal year 2020. We announced last week that we have decided to invest to increase our 2020 supply to up to 3 billion doses. Let me share with you why we decided to recommend to our board to invest at that scale. First, let's talk about the science of SARS-CoV-2 virus. New variants of concern continue to emerge around the world, and we believe that over the next six months, as the Southern Hemisphere enter its fall and winter, we could see more variants of concern emerge. We have said for a while now that we believe booster shots will be needed as we believe that the virus is not going away.
We also believe from a scientific standpoint that the highest efficacy booster over time will be provided by a multivalent variant-specific booster. The market has changed quite a lot versus what we knew six months ago. mRNA vaccines have emerged as the best-in-class vaccines, high efficacy, good tolerability profile, ability to scale manufacturing, and speed to chase variants in the clinic. Many companies are still in the clinic with their first-generation vaccine, while we're in the clinic with variant-specific boosters.
More importantly, as we are talking to governments around the world, in the West and in the East, in the North and in the South, we're hearing loud and clear from the market, "Supply us with more mRNA vaccine for primary series, and supply us with more mRNA vaccine in the future for boosters for 2022 and 2023." There is a big shift versus what the market perceived six or nine or 12 months ago, when protein vaccine or adeno vaccines were thought to be the answer to the pandemic. We believe it has become an mRNA market for COVID-19 vaccine. Third is our large pipeline.
We believe we will bring to market several more products in the next few years that will add to the market demand for COVID-19 boosters for years, flu vaccine, as we discussed at our Vaccine Day, and our goal is to have a seasonal flu vaccine combined with a COVID variant booster in a single-dose product. We also have strong clinical data for RSV vaccine and CMV vaccine. Plus, we have seven programs in clinical studies in three therapeutics area and more programs to move from pre-clinical development to clinical studies in the months to come. We decided to build capacity to deliver up to 3 billion dose of supply in 2022 to serve both the North and the South. We're doubling our drug substance supply in Europe and increasing by 50% our drug substance supply in the U.S.
We are, of course, adding filling capacity in the U.S. and Europe at our existing partners, but also adding new ones as we speak. More to come. Another piece of feedback we're hearing from the market is that Moderna has the best-in-class mRNA vaccine, shipment at -20 Celsius and storage, not -70 Celsius. Small cartons of 100 doses. Storage up to six months in standard freezer and four weeks in regular refrigerator temperatures. The only authorized mRNA vaccine that does not require on-site dilution. We believe this is an even more important feature today, but will be more in the future in 2021 and 2022 and 2023 as we move to a booster market and decentralize in pharmacies and at doctor's office. We believe we have the best mRNA vaccine authorized, and we'll continue to improve our product to continue to have the best-in-class product of the mRNA market.
We are delighted to announce this morning the start of the dosing of our first patient in our phase I/II study with propionic acidemia. The study is called PARAMOUNT. It is yet another milestone for Moderna. Not only do we have, I believe, the most innovative infectious disease vaccine clinical pipeline, but we also have therapeutics candidates in clinical studies in oncology, in cardiology, and now in rare genetic disease. Let me close my remark on this familiar slide. We now have 1,500 employees. We have recently incorporated Moderna Japan K.K., and we continue to build our commercial network with a push to Asia Pacific in 2021. Given our strong balance sheet of $8.2 billion, we're going to continue to accelerate and invest to allow Moderna to scale and maximize the impact of our broad mRNA platform to help as many people as we can.
Let me share our perspective on yesterday afternoon announcement by the United States trade ambassador that the U.S. government will support waiving intellectual property protection for COVID-19 vaccines. We believe this will not help supply more mRNA vaccines to the world any faster in 2021 or in 2022, which is the most critical time of the pandemic. There is no idle mRNA manufacturing capacity in the world. There is no industry of talented individuals who are skilled in the art of making high-quality and high-purity GMP-grade mRNA vaccines. There are no companies who have developed manufacturing processes, purification processes, analytical processes that would allow them to quickly run a clinical trial, and if approved by regulators around the world, then provide hundreds of millions or billions of supply of mRNA vaccine.
We have announced in a company statement issued October 8th, 2020, that during the pandemic, Moderna will not enforce COVID-19 related patents. You can find that statement on our website. We believe that the best way to end the pandemic is what we're currently doing. First, to maximize supply in 2021 to protect as many people as we can. Second, to build additional capacity, which we have announced last week, to get up to 3 billion doses of authorized mRNA vaccines for 2022, 2023, and beyond. Third, to continue to adapt the vaccine to have the highest efficacy vaccine with variant-specific booster, for which we announced very encouraging clinical results yesterday. Let me now turn to Corinne to give you a commercial update. Corinne?
Thank you, Stéphane, and good morning or good afternoon, everyone. As all of you already know, Moderna's COVID-19 vaccine is our first authorized product, and on the back of it, we have turned into a commercial company very quickly. Today, I'm delighted to give you an update on the commercial progress in the first quarter. I will start with our most recently signed supply agreements, those that occurred in the first quarter and at the beginning of the second quarter this year. I am particularly happy to announce our agreement with COVAX, which will provide access to our vaccine to millions of people in low and middle-income countries and is in keeping with our global access principles. In total, our COVAX agreement is for 500 million doses for delivery in the 2021 and 2022 periods.
Specifically, in 2021, Moderna will begin delivery of 34 million doses in the Q4 of 2021. COVAX will have an option for an additional 466 million doses in 2022. We are grateful to all the collaborative efforts of CEPI, Gavi, UNICEF, the World Health Organization, and the Moderna commercial teams in making this important supply agreement a reality. Moving now to the additional supply agreements signed for both 2021 and 2022. We have signed additional supply agreements with Israel for 5.3 million doses in 2022, with an additional option of 17.3 million doses for 2022 and 2023, and with Switzerland for 7 million doses in 2022, and options for an additional 7 million in late 2022 and 2023.
We have also signed new deals for 2021 delivery with Botswana, Brunei, and in addition, we have also signed an agreement with Zuellig Pharma, our distribution partner in Southeast Asia, Hong Kong, Macau, and Taiwan. In total, we announced advanced purchase agreements totaling 845 million doses to be delivered in 2021 to the countries that are listed here on the slide. We continue to have discussions with countries, both those we have already contracted with and new countries, for supply in 2022 and beyond. In our discussions, as Stéphane said, we are hearing consistently from governments that in their view, there is no other technology that provides the high efficacy of mRNA vaccines and the speed necessary to adapt to variants, while at the same time allowing reliable scalability of manufacturing.
We are grateful for the trust placed in us from the various governments we have signed agreements with, and we look forward to supplying the vaccine to other countries and helping end the pandemic and getting ahead of variants. Let me now turn to product sales. Our product sales for the first quarter of this year were $1.73 billion and were recorded for the delivery of 102 million doses. Product sales in the U.S. were approximately $1.4 billion, and sales outside of the U.S. to the EU, Canada, Switzerland, Israel, and Singapore were approximately $400 million for the 14 million doses delivered in the Q1 . In the U.S., we have successfully completed the delivery of the first 100 million doses to the U.S. government within 100 days of emergency use authorization.
We expect to complete the delivery of the second 100 million doses to the United States government before the end of the second quarter. As you know, the U.S. production started earlier and is roughly one quarter ahead in production ramp. In the second quarter of 2021, we expect the ex-U.S. ramp to be similar to that of the U.S. ramp in the Q1 . To close, I want to reiterate that as we continue to produce and roll out vaccines into the global market, we are humbled and proud to be part of the solution. I will now turn the call over to David Meline.
Okay. Thank you, Corinne
Today, as with our last earnings call, we're presenting our results primarily on a US GAAP basis. In some cases, we also provide additional detail to provide greater clarity on underlying trends. With this background, we are providing an analysis of actual 2021 Q1 results, along with an updated view of key drivers of financial performance going forward. Turning to slide 18. Total revenue was $1.9 billion in the Q1 of 2021 compared to $8 million in Q1 of last year. Following our first-ever product sales of $200 million in December 2020, we recorded product sales of $1.7 billion for our COVID-19 vaccine in the Q1 of 2021. Grant and collaboration revenue increased to $204 million in Q1, primarily due to increases in grant revenue from BARDA to accelerate development of our COVID-19 vaccine.
Cost of sales were $193 million in the Q1 , benefiting substantially from previously expensed pre-commercial inventory costs, which I will discuss in more detail on a later slide. Research and development expenses were $401 million for Q1 2021 compared to $115 million for the same period in 2020. The higher spend was driven by increased COVID-19 vaccine clinical development activities, including our announced efforts around booster, variant-specific, and multivalent vaccine candidates. Headcount increases as well as pharmacovigilance activities related to our COVID-19 vaccine also contributed to the year-on-year expense increase. Selling general and administrative expenses were $77 million for Q1 2021 compared to $24 million for the same period in the prior year. The growth in spending was driven by increases in personnel, outside services, and costs associated with commercialization of our COVID-19 vaccine globally.
Our provision for income taxes was $39 million in Q1 2021, reflecting a benefit from utilization of our net operating loss carryforward as well as discrete items. I will provide further context on the following slides. We recorded net income of $1.2 billion for Q1 of this year compared to a net loss of $124 million in the same period of last year. Earnings per share on a diluted basis was $2.84. Please note that our share count on a diluted basis now also includes the effect of outstanding options and RSUs as we began to be profitable. Previously, when we were in a net loss position, basic and reported diluted number of shares were the same. Turning to cash and selected cash flow information on slide 19. We ended Q1 2021 with cash and investments of $8.2 billion compared to $5.2 billion at the end of Q4 2020.
The increase is driven by our commercial sales and additional customer deposits received in the Q1 for future purchases of our COVID-19 vaccine. Net cash provided by operating activities was $2.97 billion in Q1 of this year compared to net cash used in operating activities of $106 million in Q1 of last year. The reversal from net operating cash outflow to cash inflow was driven by our commercial market entry for the entire quarter. Similar to last quarter, before providing an updated financial framework for the remainder of 2021, let me summarize a few areas from our Q1 results that are important to keep in mind when modeling expected 2021 financial performance. Starting with product sales on slide 20. We started last year to build two distinct supply chains, one in the U.S. and one outside the U.S. for rest-of-world markets.
Our supply chain scale-up in the U.S. was roughly one quarter in advance of our ex-U.S. supply chain, which is reflected in the geographic sales mix in Q1. As we move forward in Q2, the ex-U.S. supply chain is also ramping up toward full capability. Turning to slide 21. Cost of sales includes the cost of goods manufactured, logistics and warehousing costs, as well as third-party royalty costs. We began capitalizing our COVID-19 vaccine inventory costs in December of 2020 following the COVID-19 vaccine emergency use authorization based upon our expectation that these inventory costs would be recoverable through commercialization of the vaccine. Prior to the authorization of our COVID-19 vaccine, inventory costs were recorded as research and development expenses in the period incurred.
We expensed $242 million of pre-launch inventory costs in 2020 and started 2021 with a remaining balance of $187 million of zero-cost inventory. Almost the entire balance, or $184 million, was sold and benefited our cost of sales in Q1 of this year. Hence, it will not further impact future quarters in a material way. If inventory sold during the first quarter was valued at actual cost, our cost of sales would've been $377 million or 22% of our product sales, somewhat favorable to what we expected, driven by favorable yields in our U.S. production facilities. Now, turning to our cash and investment position on slide 22. The cash and investment balance reported as of March 31 was $8.2 billion, up from $5.2 billion as of December 31, 2020. The increase is primarily driven by the net increase in customer deposits for future product supply of COVID-19 vaccine.
The net balance of cash customer deposits increased from $2.8 billion at the end of December 2020 to $5.6 billion at the end of Q1 2021. Lastly, let me comment on tax-related items on slide 23. The significant investments in our research, development, and startup activities to develop the mRNA platform over the last decade have resulted in net operating loss carryforwards with a balance of $2.3 billion at the end of 2020. As of December 31, 2020, we maintained a full valuation allowance against our deferred tax assets related to these loss carryforwards. We perform a valuation allowance assessment during each reporting period based on the latest available financial information and outlook.
After considering the weight of available evidence, both positive and negative, we concluded that as of March 31, it is more likely than not that the company will be able to realize the substantial majority of its net deferred tax assets. This analysis included not only our strong first quarter results, but also our April activity. The majority of the valuation allowance will flow through the P&L over the course of 2021 in our effective tax rate, prorated based on the cadence of our expected pre-tax quarterly earnings. We also recorded two discrete benefits in our tax provision in Q1, which lowered our first quarter tax rate. The first benefit related to the valuation allowance release for the portion of deferred tax assets, which we expect to utilize in future years. The second related to the excess tax benefits associated with stock-based compensation.
Turning now to the 2021 updated financial framework on Slide 24. Signed advance purchase agreements for expected delivery in 2021 reflect a current full year total of $19.2 billion in anticipated product sales, including doses that have been delivered and recognized as revenue in Q1. Based on continuous progress to ramp up available supply capacity in our network, we have raised the lower end of our global manufacturing plan for 2021 from 700 million to 800 million doses at the 100 microgram dose level. Our manufacturing team and our partners are still working to supply up to 1 billion doses for 2021. We continue to expect a range of deliveries in Q2 2021 of 200 to 250 million doses. Our total cost of sales includes the cost of manufacturing, logistics and warehousing, and third-party royalties.
For 2021, we continue to model average total cost of sales as a percent of product sales to be approximately 20% for the full -year, with some variation quarter by quarter, largely driven by average selling price going forward. Let me comment on planned R&D and SG&A expenses. Q1 expenses of approximately $0.5 billion were stable compared to the underlying Q4 2020 expense run rate on a like-for-like basis. In Q1, our actual expenses were lower than the internal forecast, primarily driven by the timing of clinical development and commercial activities and related costs. We now expect a notable expense trend increase starting in Q2 on a quarter-over-quarter basis for the remainder of this year.
Based on better visibility of the utilization of our accumulated net operating loss carryforward, expected global sales mix, and the mentioned discrete benefits in Q1, we now expect our all-in 2021 tax rate to be in the low teens. This compares to our previous forecast in the mid-teen range. This forecast is based on current U.S. tax policy in effect and does not include any future potential discrete benefits related to stock-based compensation. We'll update this view as our business evolves further. Finally, regarding capital investments, we're raising our forecast for capital investment from our previous range of $350 million-$400 million for 2021 to $450 million-$550 million, including the planned capacity expansion investments as announced on April 29th. This concludes my remarks concerning financial performance, and I now turn the call over to Stephen.
Thank you, David. I'll begin with an overview of our COVID-19 strategy against variants of concern and the initial data from our phase II booster vaccine study, before ending with a summary of the rest of our pipeline. Now, before I go into the data, a reminder that our booster strategy is evaluating single-dose booster vaccinations with three different mRNA vaccines. 50 micrograms of mRNA-1273, 50 micrograms of mRNA-1273.351, both of which have data available today, and what I will discuss in just a moment, and a multivalent booster vaccine candidate, which combines a 50/50 mix of mRNA-1273 and mRNA-1273.351 in a single vaccine. In addition, we're also evaluating a lower 20 microgram dose of mRNA-1273.351. Data from the multivalent booster and the 20 microgram booster of 351 will be shared when available. Now with that backdrop, let's move to the data.
Starting with safety, local and systemic adverse events within seven days after a booster dose of either mRNA-1273 or mRNA-1273.351 were generally comparable to those observed after the second dose of mRNA-1273 in our previously reported phase II study and our phase III COVE study. The majority of the events were mild or moderate in severity, and grade 3 events occurred with a frequency of approximately 15% in participants who received mRNA-1273 and approximately 10% in participants who received mRNA-1273.351. The most commonly reported solicited local events were injection site pain, and the most commonly reported systemic events were fatigue, headache, myalgia, and arthralgia. There were no grade 4 event reports. On the next slide are figures from two papers.
The figure on the left-hand side were published in "The New England Journal of Medicine" and show the difference in neutralization of SARS-CoV-2 pseudoviruses in serum samples one week after vaccination with a primary series of mRNA-1273. Recall that there was a sixfold decrease in neutralization titers against the B.1.351 variant, the variant first identified in the Republic of South Africa, and a threefold drop in titers against P.1, the variant first described in Brazil. Again, as a reminder, these neutralizing titer levels were from serum samples one week after the second dose of the primary vaccine series of mRNA-1273. Essentially, these titers are close to peak levels. On the right-hand side of the slide is a figure from the preprint manuscript of our initial results from our phase II study, posted yesterday to bioRxiv.
The figures show the neutralization titer levels of the participants in our phase II booster study immediately before their booster vaccinations. A reminder that these individuals were previously vaccinated with a primary series of mRNA-1273 in either our phase II or phase III studies, roughly six to eight months prior to enrolling in this booster study. At this time point, titers against wild type SARS-CoV-2 remained high, with almost all participants having detectable titers. The titers against B.1.351 and P.1, the variants of concern, were much lower. In fact, approximately half of participants had titers below the assay's limit of quantitation at this time point. It is clear that waning of titers is apparent both with time and that lower titers against variants of concern lead to more rapid loss of neutralizing activity. Turning to the next slide.
The data shows that two weeks after booster vaccines of either mRNA-1273 or mRNA-1273.351, neutralizing titer levels increased against both the wild type virus as well as the B.1.351 and P.1 variants of concern. In fact, following boost, geometric mean titers against the three variants tested increased to levels similar to or higher than previously reported peak titers against the ancestral strain following primary vaccination. When looking specifically at the GMTs of the different strains, we achieved levels of 1,400 after booster vaccination with mRNA-1273.351 against the 351 variant. This compares against a GMT of 864 when boosting with mRNA-1273. Vaccination with mRNA-1273.351 was more effective at narrowing the gap in neutralizing titers between wild type and B.1.351 viruses relative to boosting with mRNA-1273.
Now, we're encouraged by this initial data, and we're excited to see additional data over time from these arms, as well as the data from the multivalent arm and the lower dose arm of mRNA-1273.351. On the next slide, I would like to highlight one last comparison from the manuscript. On the left hand is a sample of participants from the phase I study, and their neutralizing titers against ancestral strain following a primary vaccination series with mRNA-1273. GMTs achieved in this assay are approximately 1,500. On the right-hand side is a reproduction of the data we just spoke through, looking at neutralizing titers, and I'm specifically highlighting the neutralizing titers against the B.1.351 variant of concern. A booster dose of 50 micrograms of mRNA-1273, the top bar, was able to increase titers to a level of 864 in this study.
That compares with a booster dose of 50 micrograms of mRNA-1273.351, which was able to get to titers against the variant of concern as high as 1,400 in this study. We'll continue to closely watch this data, and as I mentioned a moment ago, look forward to subsequent updates and time points. On slide 31 is a snapshot of our vaccine development candidates that are in or entering the clinic. I'll highlight a few. Our CMV vaccine is on track to start a pivotal phase III study in 2021. Our Zika vaccine is expected to begin a phase II study also in 2021. Our hMPV-PIV3 respiratory combo vaccine is currently enrolling in toddlers. At our vaccines day last month, we announced positive interim phase I data from our RSV vaccine, mRNA-1345. This continues, and pediatric and older adult cohorts of that phase I study are still enrolling.
Finally, within our flu vaccine program, we expect a phase I study of mRNA-1010 to begin in 2021. Outside of vaccines, we have seven clinical proof of concept trials ongoing across four modalities. Our VEGF program, partnered with AstraZeneca, is enrolling in a phase II. Our personalized cancer vaccine program, partnered with Merck, is also enrolling in a phase II trial. KRAS, our second program partnered with Merck, is ongoing in a phase I study. Within intratumoral immuno-oncology, our phase II dose expansion in OX40 ligand, phase I triplet, and phase I IL-2 study, which is partnered with AstraZeneca, are all still ongoing. Finally, as Stéphane mentioned, we are pleased to have started dosing in the PARAMOUNT study in propionic acidemia. On slide 33, you can see our full development pipeline.
In addition to our large portfolio of infectious disease vaccines, we now have seven therapeutic programs in the clinic. I'll now turn the call over to Stéphane to take us home.
Thank you, Stephen, Corinne, and David. Our 2021 advance purchase agreement signs have now been increased to $19.2 billion. We look into 2022, we are investing to build three billion dose supply capacity because we believe the market need could be greater in 2022 than in 2021. First, we already have countries signing APAs for 2022 for additional prime series for children, but also for variant-specific boosters. Israel last week and Switzerland this morning. If you recall, they were some of the first countries to sign APAs in 2020. Again, these countries are ahead of the game for 2022 and 2023. Second, with the COVAX partnership announcement day, we anticipate to supply up to 466 million doses in 2022. Third, we're having active discussions with all the governments that have signed 2021 APAs with Moderna for new APAs for 2022 deliveries. Again, prime series, but also boosters.
We are having numerous discussions with governments that do not have 2021 APAs with Moderna because we cannot supply them in 2021, unfortunately. Many of these governments are already asking us to enter into 2022 APAs because they want high efficacy mRNA vaccines that are easy to store. This is why we decided to invest for more supply in 2022. We believe from our current deals and current discussions that the market wants more supply from us in 2022 than we can supply in 2021. As we look at the next 5-10 years, we are the most innovative vaccine pipeline in the industry, and we're investing more in research to increase our impact by bringing to the clinic more innovative vaccines against viruses that hurt humans.
We are now in the clinic in three therapeutic areas, oncology, cardiology and rare disease, and soon we should be in the clinic in autoimmune disease as well. We are continuing to innovate and invest in science like, for example, for delivering mRNA in the lung with our partner, Vertex. As we continue to prepare Moderna to scale and have 10 times more impact, we are investing aggressively. We are accelerating our investment in digital, automation, and AI. From a spend of $27 million in 2019, we invested around $60 million in 2020 in digital. We're planning to almost triple that to $170 million in 2021. We're investing across the board in R&D to ensure high quality, to accelerate the pace of learning, and to ensure we can transform clinical operations. We're investing in digital to ensure high quality, high scalability for manufacturing.
We are building commercial so that we can commercialize our pipeline in a highly efficient and effective manner. We want to change the big pharma paradigm of large, inefficient, and expensive sales force and advertising spend to promote me-too drugs. Our pipeline is first-in-class medicine that patients and doctors are waiting for. We want to enable our corporate functions, HR, legal, finance, and so on, to scale without creating large corporate organization. I'm also excited that we are launching an AI Academy. Today, we have some exciting pockets of excellence in AI across the company. AI is not yet part of our DNA. The reason is simple. Most companies don't do AI. As we grow and hire new talent, they have great skills in their art, but few have been exposed to AI in their previous company.
We want AI to be how we run the business in science, in clinical development, in manufacturing, quality, commercial, in HR, and finance, everywhere. It is the same change management revolution as 20 to 30 years ago when personal computers entered the workforce. We want every team at Moderna to understand and use AI in everything we do. AI will become part of our DNA. As many of you know, we have built integrated digital system connected to each other, and as we add more systems, we get more data. As we get more data, we learn faster, and we keep building and creating an upward cycle. Between our strong balance sheet, our mRNA platform, our team, our culture, and our digital infrastructure, I believe our ability to scale Moderna is unique in the biopharmaceutical industry. As part of scaling Moderna, there is software, but also there is hardware.
Many of you were at the opening of our Norwood manufacturing site in July 2018, or you came to visit after the opening. Our building is around 200,000 sq ft. We call it Moderna Technology Center South or MTC South. In 2020, we added the building next to it and added around 225,000 sq ft and called it MTC North. We were pleased to announce this week that we now have access to a new building, MTC East, which will start welcoming Moderna employees later this year after some investment and renovation to the building. That is another 240,000 sq ft. We now have in MTC access to around 650,000 sq ft. We now have all the building on this campus, and we can also add more buildings and build them now that we have the entire campus.
We are deeply committed about building a company that has a strong sense of responsibility. We want Moderna to be a positive force in the world, not only for our medicines, but also by who we are as a company. We are very committed to belong, inclusion, and diversity. We recently published our expanded workforce diversity figures for the first time. Last year, we signed the CEO Action for Diversity & Inclusion pledge, and we have also reiterated our ongoing commitment to increasing diversity in our clinical trials. We are deeply committed to the environment. We have decided to source our Norwood and Cambridge site with renewable energy, and we'll offset any energy that is not from renewable sources, and we will be working on a target as to when we should be a net zero carbon company.
We're also encouraging our employees to have a positive impact on the communities in which we live and volunteer. From cleaning the Charles River in Cambridge to feeding the homeless and STEM education and much more. You can find a lot of resources online on our website. As I close, I want to convey how thankful we are at Moderna to have a chance to do what we do. Every day we come to work to make innovative medicine using the first information platform of a biopharmaceutical industry. My colleagues and I work and collaborate to make more medicines to help protect or treat people. I am proud of what the team has done over the last 10 years to get us to this stage, over the last 14 months since we started chasing SARS-CoV-2 virus, and in Q1 as we continue to execute relentlessly.
As I look at the future of Moderna, I believe we have a chance over the next five, 10, 20 years to transform medicines potentially like no other company has ever changed medicine. This is just the beginning. Before taking your questions, I would like to remind you that we will be hosting our annual Science Day in a few weeks on May 27th. You are going to want to connect to this event, as Stephen and his team have some very cool new things to share with you. Later at the end of the summer on September 9th, our annual R&D Day for holistic clinical update. Operator, we'll be happy to take any question now.
Thank you. Ladies and gentlemen, if you have a question at this time, please press star, then the number one on your touch tone telephone. If your question has been answered and you wish to remove yourself from the queue, please press the pound key. Your first response is from the line of Salveen Richter with Goldman Sachs. Please go ahead.
Good morning. Thanks for taking my questions. I have a couple here. Firstly, with regard to if the U.S. supports the WTO waiver of COVID-19 vaccine IP, what does that mean for Moderna? If you could just walk us through that. Secondly, if you could just discuss contract dynamics for the vaccine in 2022 as you look to address variants and kind of we see the move towards an endemic market. Third, it's nice to see the PA program move forward. It'd be great to kind of understand whether we'll see data from that program this year and what else we might see from the ex-COVID pipeline. Thank you.
Salveen, good morning. Stéphane. Let me start with your first questions, then I'll turn the PA question to Stephen. On the IP, in term, what does it mean? I believe it doesn't change anything for Moderna. As I said, we had said last October that we'll not enforce our COVID-19 related patents during the pandemic. As I said in my remarks, there is no mRNA in a manufacturing capacity in the world. This is a new technology. You cannot go hire people who know how to make mRNA. Those people don't exist.
Even if all those things were available, whoever would want to do mRNA vaccines will have to buy the machine, invent the manufacturing process, invent purification processes and ethical processes, and then they will have to go run a clinical trial, get the data, get the product approved, and scale manufacture. This doesn't happen in six or 12 or 18 months. We have been working at this for years. As you know, there are some smaller mRNA companies that are still in the clinic trying to get the products to the finish line. We saw the news last night, and I didn't lose a minute of sleep over the news during the night. On 2022 contracts, the dynamic is as I just described it, which is the market has tremendously changed.
Since the pandemic started last year before clinical data, many countries, as you know, didn't want to move, especially because of mRNA being a new technology. They moved first on protein contracts, on adenovirus contracts, and then the kind of mRNA contracts came later, more as a just in case. The clinical data came along. The speed to get to approval. You had the fact that the proteins are still not authorized anywhere in the West. You had the lower efficacy of the adenoviruses, the safety questions around the adenoviruses, manufacturing scale-up issues that adenovirus companies have had. The big question that scientists advising governments have, which is, can you actually really boost adenoviruses with more adenovirus products?
By definition, you give, again, the same virus vector to somebody that we believe over time will get less and less response from it. As you look at the marketplace, which is what governments are doing, and given how many governments, I think last year, believed the pandemic will be gone quickly. Trust me, every government we are talking to believe this is going to stay for a long time. They have got massively educated by the scientists and the clinicians, and they believe this virus is not going away. They believe boosting is going to be critical. They believe variant-specific boosting is going to be the right way to do the science. As you saw from Stephen's presentation, this is what the clinical data are showing as well.
The dynamic is that current governments that have already contracted with us are calling for more, and we're in active discussion with all of them to supply more for 2022 and 2023, both prime series and specific variant booster. The beauty about the technology is we can agree right now in contract to give them next year the ability to choose what they want based on the clinical data, and that's an incredible competitive advantage. This we can do because, as you know, the manufacturing process is the same for 1273 or 1273.51 or 1273.211 or a new 1273 dot something if there's a new variant. We can change that on very short notice because it's the same equipment in the same room with the same people with the same raw materials.
You have all those governments, which is almost more exciting to me, that never called before, or that they called before, but we couldn't supply them because we're in the unfortunate position to tell them, "Look, we're very sorry. We have no more supply for you in 2021," which is, of course, a very difficult discussion to have given the suffering happening around the world. The great news with the investments we've announced to get up to $3 billion next year, we now can have those discussions with those governments. Corinne and her team are having a lot of discussions with them. That kind of gives you a sense for the dynamic. Stephen, on PPA?
Sure. Salveen, thanks for the question. As you know, the program, the PARAMOUNT study for propionic acidemia, is going to be looking at biomarkers as a part of its dose optimization. It's possible that we'll be seeing very early indicators of impact there. There's no guarantee that the first dose level and the first cohort that we're looking at will be the correct one. We're going to make sure that we develop a cogent and consistent data set before we bring that forward. It is a dose optimization study, and we will perhaps be looking at multiple dose levels. While I think it's possible that we would see data this year, it's dependent upon many things that are well beyond our control. Now, you asked a more general question also about our broader portfolio.
If you look at the programs more generally, VEGF, as we mentioned, is a phase II program that's been enrolling for a while. It's possible we could see data from that. Our PCV and KRAS programs, again, as open label programs, we'll continue to track those closely as they enroll. Similarly, the intratumoral programs that we highlighted, many of them are ongoing and producing data. Of course, when we have a complete and cogent data set, we will bring it forward.
Thank you, Savine.
Thank you. Your next response is from Matthew Harrison with Morgan Stanley. Please go ahead.
Great. Good morning. Thanks for taking the questions. I guess two from me. One on the sort of next generation COVID-19 vaccine where you think it might be refrigerator stable. Can you just talk about the regulatory path for that vaccine, given that it's not the full spike? Do you think you might have to run an actual efficacy study, or do you think a neutralization titer study with safety might be enough for that? The second question, Stephen, if I can just follow up on PPA. I know in the past, one of the struggles has been enrollment. Obviously, it's great to see that you've gotten a patient into the study. Can you just talk about now that you've gotten a patient in, what your sort of view is around enrollment, and if you think you've gotten through some of those hurdles?
Thank you, Matthew, for both questions. First on, I believe you're referencing our second-generation vaccine candidate, which is mRNA-1283. It is a shorter construct that we think could have a much longer refrigerator stability profile. As we announced previously, we've started enrolling in the phase I in that study, it's probably a little bit premature to comment on what we think the regulatory path will look like for that until we get some of that initial data and have conversations, obviously, with regulators. I would highlight that it's possible that 1283 may not go into a full primary series vaccination study. It could, in fact, in the future, function as a booster. Again, that's probably too early to say. We would have to wait until we see that data, ultimately it would be dependent upon conversations with regulators in the future.
As it relates to PA enrollment, yes, as you mentioned, we've been working very hard on that over the past years. We're quite pleased to have enrolled the first participant, the first patient in that study, and the team's working hard to enroll additional patients as quickly as possible. I think time will tell whether we've actually broken through here and addressed any of the issues that we previously had in terms of enrollment. Hopefully we'll be able to provide subsequent updates on expanding enrollment in the near term that will demonstrate that we've made that progress.
Thank you. Your next response is from Ted Tenthoff with Piper Sandler. Please go ahead.
Great. Thank you very much, thank you for all of the detailed updates, including running through the financial analysis. So detailed, David. Congrats on all the success. Stéphane, I wanted to pick up on the booster data that you've shown, maybe you can take us a step forward. What is the booster strategy going to look like? Do we actually need to maybe redose or revaccinate sooner than eight to six months because of where the levels were? Maybe you can just tell us what you see as sort of the potential timing for when we will be getting boosters. Thank you.
Thank you for the question, Ted. Look, I think we have to start by saying we don't know. We do not have data on when to expect waning immunity leading to breakthrough infections. We do know that there is a raging pandemic, that reinfections will happen at some point. The best way to ensure that we do not have renewed outbreaks in well-vaccinated countries is to boost and maintain the highest possible levels of neutralizing immunity. We, as Moderna, also believe that that means we want to maintain the broadest neutralizing immunity against the largest number of then-circulating variants of concern.
If you look at the data that we have posted today, as well as some of our published data and others' reports, it does feel like immunity to a primary vaccination series or a previous infection seems to wane over the six to 12 months time horizon, at least as measured by neutralizing titers. We don't know whether that's a clinical correlate or not, but it certainly is an indication of that waning immunity. If you look at the data that I presented earlier, approximately half of the participants in our booster studies no longer have detectable neutralizing immunity against the variants of concern. They have neutralizing immunity against the ancestral strain that they were vaccinated against.
The logical thing we think to do is to boost their immunity against those variants of concern, if you will, vaccinate them against those to both increase those titers right now, but also give them a longer duration of protection, perhaps long enough that we can see our way through the pandemic. That probably looks like boosting on a 9 to 12 months after primary series as an annual booster for now, at least while we're continuing to see the evolution of the virus. The last point was about our strategy more generally here.
We do believe that the virus is not going to follow one path of evolution, that we are going to see many variants of concern, that there may be divergent paths, and therefore the best way to ensure that we can protect against the broadest number of variants of concern will be a multivalent vaccine. Now, right now, we're still waiting to see our multivalent vaccine data, which is a combination, as you know, of ancestral and B.1.351, or the strain first identified in South Africa. We think this is just the beginning, and we think we're going to be unfortunately continuing to fight this pandemic through 2022, at least globally.
Therefore, we're committed as a company to make as many updates to the vaccine, to add as many variants as we think are necessary to ensure that when people receive a booster, it provides the broadest immune protection against the widest range of variants.
Incredibly helpful. Thank you guys for all the work you're doing.
Thank you, Ted.
Thank you. Your next response is from Michael Yee with Jefferies. Please go ahead.
Hi. Thank you. Good morning. Appreciate the questions. I had two important follow-ups. One was going back to the question about the WTO. Can you just offer some color around the view of raw material supply capacity, et cetera? In other words, shedding some light on any ability to actually increase global capacity, even if there were some form of open patent. Maybe just talk about that, because I don't think that you can just make it. I don't think it's that easy. Can you maybe just offer some color there? The second question is also a follow-up on the variant strategy. It sounds like the bivalent strategy might be the best, Stéphane. At what point would you just pull the trigger on beginning to manufacture that and ramp that all up for 2022? Thank you.
Thanks, Michael. Stéphane, let me start on the raw material and the IP. Going back to what I said, is that if somebody was to start from scratch, because again, there is no mRNA player.
That's with idle capacity out there. One will not start by focusing on large-scale raw material supply. I mean, one will have first to figure out how do you make mRNA, and you cannot find that in our patents, which as you all know, are on the internet, on the U.S. patents office website. One would have to figure out what machine do you need, how do you make mRNA, what purification method you need, what analytical methods you need. Once you have figured out all those things, which trust me, is going to take you time, it is not easy. Us and other companies that are on the market with mRNA vaccine have been working on it for decades.
Even companies that have been working on it for 10 or 20 years are still in the clinic trying to figure out how to get to the finish line. I really believe that this is not the issue. I really believe the IP topics is mostly politically driven. This is not the issue. It might impact other technology like adenoviruses and proteins, and this I could not comment on. For mRNA, I really think this is the wrong question. Stephen, you want to take the baton?
Yeah. Thank you again, Michael, for the question. On our multivalent strategy, at this point, we're still waiting for the clinical data to confirm that. We expect to have that shortly. As we've mentioned, we previously dosed people with the mRNA-1273.211 variant. The preclinical data that we have published or presented, it does suggest that that is going to be the winning approach. As I highlighted, or as is highlighted by the monovalent clinical data we already have, there is a benefit to adding additional antigens, and potentially therefore benefit with a multivalent approach. I think it's important to recognize that we view this as an ongoing battle.
Your question about when do we pull the trigger and move forward bivalent manufacturing, we're already on the path of doing that manufacturing, not because we think that we're done with mRNA-1273.211, the current bivalent vaccine, but because we think we are going to go down the path of multivalent vaccines and continue needing to add things. That platform capability, we are already in the process of building and establishing to support multiple updates to a multivalent vaccine. We do think that's going to be required because we think the virus is not going to stand still and stop evolving, and we suspect there's going to be trivalent, maybe quadrivalent. It will keep happening in the time ahead.
We have completed GMP manufacturing of all of those batches, and we're at sufficient scale, we think, to be able to quickly move into commercial scale distribution if needed. At this point, we are still waiting for data to come shortly to confirm that performance in the clinic.
Just a point to add to Stephen on the multivalent, which I think a lot of people don't appreciate, is it is not easy to do a multivalent mRNA GMP product from an analytical QC standpoint, because those mRNAs are the same size. They look mostly similar because you just change a few nucleic acid. That takes time, and it's where the platform comes to drive so much value. As you all know, we have a CMV vaccine on its way to phase III, where we developed, and as we find over the years, a very complex product, six mRNA in the same vial. If you think about what the multivalent vaccine for COVID is going to look like, it's not going to be easy.
For people that have not done multivalent in GMP setting before, trust me, the regulators, because we've had this discussion with regulators around CMV over the years, they're going to want to see a lot of analytical method characterization so that you can prove to them that you know what is in the vial. That is yet again, another big differentiation with Moderna. Thank you, Mike.
Got it. Thank you, guys.
Thank you. Your next response is from Gena Wang of Barclays. Please go ahead.
Thank you for taking my questions. I also have two, one also related to the IP question. Wanted to ask differently. Just wondering, Stéphane, how many contract global manufacturing sites you have, and how long in general is the contract? The second question also regarding the new booster data. This more for Stephen Hoge. Actually, to me, I was a little bit surprised the differences between mRNA-1273 versus mRNA-1273.351 was less or narrower than initially I would expect it. Seems like mRNA-1273 should be also sufficient to protect from a varying strain. What could be the explanation? You did just lay out the plan. You still will be going after the multivalent approach, but regarding the explanation there, do you think that just single shot that was dose should be sufficient for the protection?
Thanks, Gena. Maybe I'll take that question first and then hand it back to Stéphane on your IP question. A couple of things I would note. The first is the level of titers, as you suggested, there's a little bit less than twofold difference between them. You are seeing substantially higher titers, on the order of 1,400 when you give the variant specific booster, that's mRNA-1273.351. I would note that this is happening already at day 15. This is an early time point that we're looking at at this point. We will also be looking at day 29. In this case, effectively it is a prime with the mRNA-1273.351. It is the first dose of the strain first identified in South Africa. Actually, there's two ways you could look at it. One is obviously that it is both look good.
I think the other, and the way that I'm still looking at this is, it looks like we can very rapidly direct the immune response to an increased level of neutralizing titers against the variant of concern that was first identified in South Africa, 351 in this case. If you compare the titers that we've achieved, even by day 15, between these two variants, or between the ancestral strain and the 351 strain, it's really only the 351 strain that's getting to the same level that we saw against the ancestral strains in that last comparison. To levels that are approximately similar in amount. Now, that's not to say that mRNA-1273 as a booster wouldn't provide a benefit, and I think you're highlighting that, Gena.
There is evidence in this data as well, that we can substantially increase neutralizing titers generally across the response with a booster dose of mRNA-1273, our authorized vaccine, at 50 micrograms. That is encouraging. That is good news. I think it suggests that is also a useful strategy. If you had to choose between the two, and you were primarily concerned about increasing immunity to a higher level so that it can last longer, particularly in patient populations at high risk of either waning immunity or incomplete immunity, we think this starts to provide very early evidence, even at day 15, even after a priming dose, that there's going to be an advantage to some strain matching of the antigen. That's what has us continue to be excited about a multivalent strategy. Stéphane?
Sure. Thanks, Stephen. So Gena, on the contract manufacturers, we look at raw material, drug substance and drug products. In all of these, we have multi-year contracts. As soon as we got board authorization to go to a 3 billion supply for 2022, we right away sent a lot of orders and a lot of additional supplies to our suppliers. Not to forget the drug substance, actually, now this is the place, it is on Moderna site, where we actually have the biggest capacity of drug substance, even in the 3 billion dose 2022 scenario. Over.
Thank you.
Thank you. The next response is from Geoff Meacham with Bank of America. Please go ahead.
Good morning, guys. Thanks so much for the question. Just have two on COVID. The first one is, what does your data tell you, with real world effectiveness of 1273 today, as of now, with respect to some of the main variants? I'm just trying to reconcile the need for annual boosters versus minimal breakthroughs thus far, and high efficacy. The second question is, when the next-gen vaccines for COVID-19, when you have some permutations, what's the potential to leverage the technology to use different parts of the virus versus just modifying the spike protein? Do you think this could add regulatory steps that make it difficult, even if it's theoretically possible? Thank you.
Thank you, Geoff. Those are both good questions. Maybe I'll take the first one. First, which is our real world evidence, the largest amount of it that we've seen has been published by groups like the CDC, and continues to reinforce that the efficacy we saw in the clinical trial seems to be translating well into real world use, with very high efficacy, against COVID-19. I think it's important to note, though, that this is all happening very acutely, right? We're still only months into these vaccination campaigns. The primary concern that we and others have from a public health perspective is really not what's going to happen right after vaccination, but what does this look like in nine months? What does this look like in 18 months?
I think the really difficult situation everybody is in is you could say, well, let's wait until it's a year from now, and we see in a reemergence of spikes of cases, we see maybe it's not as bad, but we see a very big and bad flu season in the winters. Tens of thousands, maybe hundreds of thousands of deaths, that kind of scale. That's not a situation that most are willing to take a risk on. It obviously could be substantially worse than that. We're probably not going to have a chance to wait for data for cases to really break through a year from after vaccination in the real-world setting and let that start to guide revaccination decisions. At that point, it's almost too late.
I think at this level, we think for the very near term, the correct and sort of conservative decision is to continue to try and maintain the highest level of broadest immunity in the populations that are well vaccinated already. Now, if you look beyond this sort of epidemic phase or pandemic phase that we're in with this variant evolution into the years beyond that, so, three, four, five years from now, hopefully we're well past the current pandemic, we still believe there's going to be SARS-CoV-2 reinfections. As we shared at the vaccines day just a couple of weeks ago, we take that lesson from the previous endemic coronavirus epidemics that have happened, where hundreds of years later, you still see reinfections, mortality, substantial healthcare costs associated with those viruses.
We don't know whether SARS-CoV-2 is worse than them or the same, but we believe that that burden of disease that's created by the fact that respiratory viruses continually reinfect, and when they do, they can really have a devastating effect in high-risk populations, particularly older or immunocompromised. We think that's a real probability in the future. In fact, it would almost be unprecedented for that not to be the case in the coronavirus context. For that reason, we believe there is going to be a need for continual boosters. Whether it's annual or not, and whether the multivalency continues to add more and more valencies, I don't think anybody can say yet. It's certainly a situation we're preparing for.
Stéphane, just on the second question on the different modalities, or Stephen, either one.
Yeah, sorry. I apologize. Your second question on different modalities and different parts of the antigen. I think what is pretty clear from all the vaccines, if you look across them, but certainly if you focus on the messenger RNA vaccines, is that the high degree of efficacy we're seeing in vaccines right now is based on spike protein immunogenicity. That is the antigen that's being expressed. Is it theoretically possible that non-spike antigens could have provided the same protection? I think it's definitely possible, still forward-looking possible. I think you would be remiss to look past the multiple large phase III trials that provide pretty conclusive evidence of the value of going after spike protein and trying to prevent COVID-19. I think you would probably, if you went down that route, have to re-demonstrate that efficacy.
That may be increasingly difficult in a world where we have so many good choices in terms of vaccines. I'm not exactly sure how we go down that path, even theoretically.
Okay, great. Thank you so much.
Thank you. Your next response is from Cory Kasimov with JPMorgan. Please go ahead.
Hey, good morning, guys. Thanks for taking the questions. I want to go back to the topic of the future contracts. I know this has kind of been asked in a couple different ways, but based on discussions and negotiations that are currently taking place, how much confidence do you have that there's going to be demand to fill up to 3 billion doses in anticipated supply that you think you could have next year, especially if people are getting a single annual booster in the future? The second question is from really a modeling perspective, are the price points currently being negotiated on future contracts comparable to what you have on the existing ones for 2021? Just basically wanted to see if we should be assuming stable pricing for modeling purposes for 2022. Thank you.
Yeah. Let me take a stab at the question, Cory. Anytime there's anything, Cory, just please add some color. As I said in my remarks, from what we're hearing from customers, this is becoming an mRNA market looking forward. There are not so many players in the mRNA market. If you look at what the future needs across the globe, you're going to still have to vaccinate adults. All adults are not going to get vaccinated this year on the planet. The math doesn't work. You have adolescents, and then you have children across the world, and then you have boosting.
When you add all those pieces, the reason we are building up to 3 billion of supply as a mix between prime series and boosters is because we believe that this is what the world is looking for based on the daily engagements we have with governments around the world. It's a very different setup than what it was a year ago. A year ago, you had people saying, "Oh, I'm going to get a cheaper adenovirus vaccine because it's being supplied at cost." This is not the discussion anymore today. The discussion is, "I want some mRNA vaccine for all my people. I want variant booster specific. I want multivariant. I want the best thing because I don't want a second and a third and a fourth year with this thing. I need my country to be back on its feet." Make sense, Cory?
Yeah. No, on the pricing question for modeling purposes.
No, look, I think I won't give you any color. Again, there is no more discussion at all that your price is this, and there's a small company, there's a company with price at cost of $3. This discussion is gone.
Okay. All right, perfect. Thank you, Stéphane.
Thank you.
Okay, your next response is from Hartaj Singh with Oppenheimer & Company. Please go ahead.
Great, thank you. Thanks for the question and all the color. A question I would have is on the 50 microgram going forward, as a booster and against variants. Would you see that potentially becoming your initial prime boost vaccine possibly in the future? Do you think you'll stick with the 100 microgram route, whether it's with mRNA-1283 also? On OpEx, any color when we think about 2022 and 2023 for David Meline, what cost of goods sold could look like, once these quarterly variations flush out, and what your adjusted operating margins could start looking like also. Thank you for the question.
Thanks, Hartaj. I'll take a stab at the first one and then hand to David for the other. On 50 micrograms, obviously the data we shared today is a small number of subjects. We previously shared and published our phase II data on a slightly larger number of subjects looking at a 50 microgram primary series that looked quite good, and at least as measured by immunogenicity, seemed to achieve levels that were consistent with the 100 microgram dose. It's certainly something we're going to look at as to whether or not we could pursue a 50 microgram primary series. How we get there will depend upon data that we don't yet have, right?
We will have to look at whether there are clear correlates of protection that we can use to bridge between those doses, and/or we'll have to look at different populations in which we study those doses. As an example, as has been shared, we're evaluating 50 micrograms as a potential primary series, even in pediatric populations. As you can imagine, you don't need perhaps as high a dose in younger people than you do in older ones. There's a lot of things ahead of us in terms of looking at whether or not a primary series for 50 micrograms is possible. Certainly for a booster series, that is the top dose at which we're looking. As we look forward, we'll continue to carefully evaluate whether or not we can adopt that as a target dose across all of our applications. It'll depend upon data.
Great. Just a question on.
Go ahead.
Thanks, Dave. No, go ahead, Dave.
Yeah. Cost of goods and operating expense trends as in 2022 and beyond. I guess what I'd say is it's a little early to start giving that kind of guidance for 2022. What I would say is that if you look at our cost of goods and the cost of goods manufacturing thus far, we've been quite pleased with what we're seeing as we've ramped up production initially here in the U.S. We've seen, as I said, yields have been better than we'd foreseen as we did the initial planning, so that's obviously very helpful. We reiterated today, we think right now the right planning assumption continues to be 20% cost of goods. As you move beyond 2021, you get into a question of vaccines versus therapeutics.
We think cost of goods manufactured will be very competitive for this product, and therefore margins, and gross margins will depend very much on price levels, which I think it's early to comment on. In terms of operating expenses, we are building out the company. We continue to do that, and as I mentioned, while our overall operating expenses were quite stable at a half billion in the first quarter, we do see that trending up and move through the year. We'll continue to invest appropriately to drive the portfolio investment and to build out globally. I would say we'll continue to do that, and we'll give you better and more precise guidance here as we move closer to 2022 and beyond.
Great. Thank you.
Thank you. Your next response is from Joseph Stringer with Needham & Company. Please go ahead.
Hi. Good morning. Thanks for taking our questions. Just another one on manufacturing capacity here. As you potentially move to next generation COVID vaccines, I was wondering if you could give us a sense, maybe even qualitatively in terms of given the modularity of the technology, what a potential manufacturing ramp would look like for some of these second-gen vaccines in terms of manufacturing capacity and the ramp relative to what we had seen with 1273. Thank you.
Yes, it depends. The 1273 ramp has been constrained by manufacturing capacity. If you look at this year, the only reason we quote-unquote, only supply $100 million to those in Q1, which is an extraordinary number, is because we are building the capacity. The way to think about it is as Juan and his team are working hard to add new lines and to increase the capacity, the ramps of the follow-on products will be much faster, because today manufacturing is slowing down the ramp. I anticipate that as you think about the multivalent booster launches, as you think about RSV, flu, CMV launch, we will not be on the back foot here. As you know, as part of our 2020 budget that we did at the end of 2019, we did not plan for a pandemic.
We were supposed to be commercial several years down the road. The team has done a remarkable job to get to this point. We are, and we're going to stay for, I would anticipate all of the year, supply constraint. Corinne and her team would love to product, because trust me, their phone is turning red-hot by calls from around the planet. We would love to be able to help protect more people, but we just can't because we were not planning on a pandemic in 2020. I anticipate that for variants and for new product launch, we will make sure that we're not capacity constrained, which is why the 3 million supply volume that some people might think is maybe too aggressive.
As I said in my remarks, the pipeline of a company is also going to play with this. Flu is just behind the multivalent vaccine. If you look at a couple of years out, and we're not building manufacturing for six months, we're going to be really happy to have that capacity so that as we launch product, we can supply the market every single dose that Corinne and her team can make sure that the market wants. Over.
Great. Thanks for taking our question.
Thank you.
Thank you. Your next response is from Mani Foroohar of SVB Leerink. Please go ahead.
Hey, guys. Thanks for the question. One quick one, starting on financials. You gave a little clarity on CapEx investments around expanding capacity and production. Should we think of that as level setting CapEx going forward, with a modest increase going forward, or should we think of that as primarily a one-time build-out? Secondarily, you've given a little bit of clarity, in fact, a lot of clarity, around COGS for this quarter versus the rest of the year. Going forward, should we think about the absolute COGS per unit as being pretty linearly related to dose? Are there other attributes, royalties, et cetera, differences in product use between different vaccines. Would that suggest that that's not the right way to think about it?
Yeah. CapEx, if I understand the question, is guidance for 2022 and beyond on CapEx. Again, unfortunately, it's a bit early to be able to comment. If you look now, we've increased our guidance for this year based on the developments that have occurred over the last couple of months. Is that a steady state going forward into the future? I think for a company of what will be our size and scope and level of vertical integration, I think it's reasonable to expect that we'll continue to invest in our own capacity, and therefore, you can expect we'll have ongoing CapEx. Is it precisely in this range, or somewhat above or below? I won't give you that precision as of yet because it's not that clear yet. I think an ongoing CapEx trend will be appropriate for the company.
In terms of COGS, I think that's the right way to think about the cost of manufacturing this product. It will be impacted at some level by the amount of materials in the product. When you're talking about micrograms of materials, it's really not that significant. I think you can, as the simplest assumption, assume it's a pretty steady cost of manufacture as we're seeing right now.
Great. A quick follow-up on some of the contracting. I know it's been asked in various forms. You talk about the OUS manufacturing being about a quarter-ish behind U.S. manufacturing. Right now, based on your disclosures on volume, doses delivered, versus what we have from the other mRNA competitor in the duopoly right now, market share for you guys is somewhere between 5% and 10% in a lot of these countries in terms of delivered doses. How do you think about catch-up on manufacturing and deliveries, and how that influences the ongoing contracting for next year? I.e., does your stronger incumbent position in the U.S. suggest you have better positions for U.S. contracting volume? Do you think the results for 2021 contracting have nothing to do with 2022, and every year is a whole new game between you two?
Yeah, it's a good question. As we said, the plan was always the plan we're executing on. When we spoke to countries and set up those 21 APAs, delivery to the countries as we knew capacity would come online. We had not anticipated the early part of the year that we'll be able to export from the U.S. We should not forget is, as you know, it's reported in the media daily, the U.S. is going to be having way too many vaccines very soon, if not already. The capacity that we are building in the U.S. and we are adding is also going to supply the world.
I think one should anticipate a very big acceleration of shipments to countries outside the U.S. as we go into Q2 and even more in Q3 and Q4 as we met our obligation to the U.S. government.
Great. That's really helpful. Could you guys comment on what you're seeing in the real world in terms of vaccine hesitance/end market demand? There's been a couple of media reports that that's starting to be more of a limiting factor as opposed to supply, at least in the U.S. currently. Certainly not globally.
Correct. In the U.S., you see it on the daily numbers provided by the CDC for, I think since mid-April, roughly, the number of vaccinations per day in the country is going down. It is not because of supply. If you look at the shipments coming from the companies, they keep increasing exactly as we have been saying. Now there's an oversupply of vaccine, and it's really a demand-driven problem now. You see some states across the country that are still very active in vaccination, and we're lucky in Massachusetts that our very high rate of vaccination is going up every day. There are other parts of the country, as you know, where they have way too many vaccines.
You have heard that the federal government, I think yesterday or the day before, has announced that they were going to start to shift product from one state to the other based on actual demand, and it is relating to vaccine hesitancy. The U.S. doesn't have a supply of vaccine issue anymore. That was true in January. It's not true anymore.
Great. Thanks. I'll hand back to you in case there's others.
Thank you.
Thank you. Your next response is from Simon Baker with Redburn. Please go ahead.
Thank you for taking my questions. Firstly, just going back to the debate around IP waivers. Stéphane, as you said, the talk of IP waivers rather misses the point about limited global manufacturing capacity. To sort of support that, given that you announced you would unilaterally waive IP enforcement back in October, to your knowledge, has any company in the following seven months sought to exercise that freedom to operate? Also sticking with the vaccine, going back to slide 28, do you have data on T-cell response over that six to eight-month period post primary vaccination for 1273? Then just a quick question on the financials. David, I think you mentioned that in the SG&A in Q1, there were some costs related to effectively startup of supply.
I just wondered if you could give us any color on the non-recurring one-off elements of SG&A in Q1 as we think about evolution across the year. Thanks so much.
Thank you so much. I'll take the first one on IP, and Stephen Hoge will take the T-cell, and David, the financials. I'm not aware of any company offsite that is going after mRNA. Again, going back to what I described, one will have first to figure out how to make mRNA GMP before asking the regulators to start a clinical study, and that doesn't happen quickly. We are monitoring the field very closely, as we always have. At this stage, I think that this is really not the point that will impact, of course, 2021 if possible, but even 2022. Stephen, on T-cell?
The data we have from today is the initial analysis on the boosters. As we previously announced, we've been looking at a study ourselves and with the NIH. They're running a primary series vaccination study and also looking at other elements. We will perhaps get T-cell data in the midterm. At this point, we do not have any T-cell data yet on the boosters. We do have ongoing studies with NIH in general on our phase I and our own phase II. If it becomes important in the future, we can obviously look at T-cell responses with waning immunity out six, 12 months in those studies as well. We don't have a specific plan to do that at this point.
We are pretty encouraged by the historical correlation between our previously reported T-cell data and published T-cell data and the neutralizing titers that are obviously much easier to measure over time across a wider range of subjects than T-cells.
David?
In terms of the expenses we incurred in the first quarter, including in commercial, we had some one-time expenses to set up businesses around the world. But the preponderance of the total operating expenses, including in commercial, I would say, will continue. That's why I gave you some guidance that if you start at that half billion spend level in the first quarter and the fourth of last year, we're expecting now to see that trend up notably as we move forward through the year, which we thought it would start sooner, but we now expect will start in the second quarter. Running a business of this size on a global basis, we think that that spend level is quite reasonable, to be honest.
Great. Thank you very much.
I'm showing no further questions at this time. I would now like to turn the conference back over to Stéphane Bancel.
Thank you so much for participating in this call and for the great questions. We look forward to seeing you at Science Day on May 27th. Stay safe, everybody. Have a nice day. Bye.
Ladies and gentlemen, this concludes today's conference. Thank you for your participation.