Hello, everyone. Welcome to today's live broadcast, the Agilent Cannabis Virtual Learning Lab, a context rich interactive knowledge base. I'm Megan Metzano, I'll be your moderator for today's event. We are pleased to bring you this webcast presented by LCGC and Cannabis Science and Technology and sponsored by Agilent. I would like to share a statement from our sponsor.
Agilent is a leader in life sciences, diagnosis and applied chemical markets. The company provides laboratories worldwide with instruments, services, consumables, applications and expertise enabling customers to gain the insight they seek. Agilent's expertise and trusted collaboration gives customers the highest confidence in the company's solutions. We have a few important announcements before we begin. This webcast is designed to be interactive and we encourage you to ask questions during the event.
You can submit questions by typing them in the Q and A box, which can be found directly below the video player. You can enlarge the slide window by clicking on the small icon in the bottom right corner of the media player. The slides will advance automatically during the event. If you have any technical problems viewing or hearing this presentation, please click on the question mark help widget in the top right of your presentation window. I would now like to introduce today's speakers, Heather Reese and Jean Francois Roy.
Heather brings over ten years of experience serving the cannabis industry. She started her career as a chemist with a leading manufacturer of products for crop production and pest management. Shortly thereafter, she made the transition from the bench to the field as an analytical instrumentation technical support specialist. Located in Northern California, she has been on the forefront of cannabis testing for years, providing a bridge between applications and implementation. As Agilent's National Account Manager for emerging markets with a current focus on cannabis and hemp, she interacts with new and existing customers daily to help them understand their current efficiencies and plan for the future.
Heather's goal is to make the process of testing as simple and efficient as possible, while maintaining a foundation of sound scientific principles. Jean Francois has worked at Agilent for fifteen years, focusing on chromatography and mass spectrometry. His work revolves around developing applications requiring mass spectrometry detectors with a focus on the Canadian market. He is located in Montreal, Canada and has recently devoted much of his time to understanding the challenges of cannabis testing in various matrices. Thank you both for joining us today.
Please get us started.
Hello and thank you for joining us today to introduce the culmination of two years of effort, the Agilent Cannabis Virtual Learning Lab, a context rich interactive knowledge base. For today's agenda, we will be talking about why this Virtual Learning Lab was created in the first place. Then we will take a walk through it together where you will find helpful items for new and established labs alike. Please stick around to the end where you could gain access to some giveaways. Finally, to continue to support the industry, Jean Francois Roy will take over and discuss some applications that Agilent has been working on.
All right, let's get this party started. First, a little background on why this tool was created because this industry was brand new, it really was one of the first where a holistic approach has been necessary to ensure success. It is common to have hours long discussions about build out, the space these workflows can take or require, and vendor considerations for cannabis testing labs. Sometimes this can be difficult to communicate and understand without a visual framework to reference, especially if it's the first time you're building a lab. Because this need comes up so often, we wanted to develop that visual framework also referred to as a layout.
For those who already have some understanding of the layout, we often dig a little deeper. Some other questions that might come up are things like, what should go in each room? What are the spatial considerations for instrumentation and equipment? What do these items need to operate? The final few questions center around resources and where to find them.
These include questions such as, what's the best way to get me up and running as quickly as possible? And will you continue to support both my lab and the industry with the latest information or application notes? Because we constantly strive to produce new information, we now have a centralized resource hub that is updated regularly. This approach makes it easier to find essential information as compared to more traditional vendor webpages. Taken together, these elements comprise the Agilent Cannabis Virtual Learning Lab, a multifaceted online resource that aims to address all the items mentioned above in one easy to follow interactive visual space.
It's a great place to go when you want to dive in and see what an actual bench space or instrument looks like while navigating through a central library for up to date application notes, brochures, ordering guides, informational videos, and more. I do want to mention that while we will only briefly discuss lab layout today, if you would like more detailed information about that, please reach out to cannabis.enquiriesagilent.com where a link to access a previous webinar about that can be provided. Now let's take a tour of the space and discover the resources it contains together. Now when you open the Agilent Cannabis Virtual Learning Lab you will come to this screen. On the left hand side you see a few options to navigate including the lab floor plan which is where we are now, potency and pesticides testing, heavy metals testing, residual solvents and terpenes testing, and microbial testing.
These three bars at the top simply toggle between viewing those panes and not viewing these panes. You will notice that the panes on the left correspond to the blue highlighted rooms of the lab floor plan. That is because if you click on either the pane to the left or the room itself, it will take you inside of that room for you to navigate. We will be doing this together shortly. Now this generic lab layout will be of interest to new labs, labs looking to add testing capacity, or those looking to bring testing in house.
If you are an existing lab or already an expert in the field, stay with me for a little while longer while I briefly discuss this layout. While there are many possible layouts for a cannabis testing laboratory, the goal of this layout is to bring awareness to the special spatial considerations for cannabis testing and is, as with any example floor plan, open to your interpretation and specific needs. Please do consider a consultant before signing off on any lab space or layout design as this tool is for demonstration purposes only. The layout you see here takes a hub and spokes approach with sample preparation ultimately being centrally located. You'll notice the vault room where the samples are stored are near the sample prep lab as well as the location for sample intake, accessioning, weighing, water activity, and moisture content.
Most likely you'll be wheeling carts around with raw samples that can take up a lot of space and can be a hazard. Thus it would be wise to minimize the distance these carts need to travel anywhere in the lab. The miscellaneous lab toward the back could be used for things like dishwashing and autoclave, storage stability chambers should you choose to offer that service or any other service not shown here. Everything from the sample intake room to the miscellaneous lab should have a special keyed entry. The utility room and storage are found in the back where the special access may not be necessary.
A roll up door for ease of deliveries and waste disposal is optimal. Let's now get into some of the interactive features of this virtual learning lab. So if I click on the potency and pesticides room, I will physically enter that room. Once inside, you will notice the buttons at the bottom. The plus sign zooms in on the room while the minus sign makes your view smaller.
At any time you can click and drag your way around the room in three sixty degrees. Each of the instruments found within these rooms is shown to scale as AutoCAD files were used to prepare this tool. Hovering over each instrument is the workflow and associated instrument model name. As we make our way about the room, we see the Agilent twelve sixty Infinity II LC system with the Ultivo Triple Quadrupole Mass Spectrometer for pesticide analysis. A quick note, pesticide testing by LC MSMS is required in every state.
Second is the Agilent twelve sixty Infinity II LC System for potency testing. Third, we have the Agilent Intuvo 9000GC with the 710C Triple Quadrupole mass spectrometer for pesticide analysis. Please note that this may not be required in every state depending on your compound list and associated action limits. You will want to check with your local Agilent representative to be sure. For the GCMS instruments located throughout this tool, gas is required for all but not shown due to individual preferences on how to plumb the gas such as utilizing tanks next to the instrument or from a centralized source.
And finally, we have the Agilent Intuvo 9000GC with the 5,977 single quadrupole mass spectrometer for terpene analysis. Now these blue hotspots are where you can find additional resources and details about each workflow. Clicking on the hotspot on each instrument will pop up a short video explaining the instrument and associated workflow such as potency testing.
The Agilent twelve sixty HPLC is the proven platform for potency testing of cannabis and hemp. Complete with potency kits, standards, reporting templates, this solution will get your laboratory up and running quickly. The ease of maintenance of the twelve sixty HPLC and demonstrated robustness minimizes downtime and keeps the lab running smoothly. A variety of sample preparation methods are available based on your sample type, including the flour, gummies and hard candies, beverages, chocolates, and fat or lipid rich products.
Clicking the X at the top will take us out of the video. If I click on the blue hotspot for the monitor, this is where the additional resources can be found. Notice we have application notes, webinars, videos, flyers, and websites all in one spot and all specific to each application. If I click on this blue hotspot associated with pesticide testing, you will find different resources that are specific to pesticide testing. Please note that these resources will be continuously updated so it would be a good idea to bookmark this tool.
Additionally, we have tried to incorporate other necessary items to each room for your consideration. For example, here you will see rough pumps on the floor under each mass spec instrument. Not shown is the tubing that connects the rough pump to the instrument. This is what pulls the vacuum. While monitors are shown, that PCs are required.
You will also notice a nitrogen generator under the LCMS triple quadrupole instrument to supply the appropriate gas. If you need additional information about size, weight, power, and gas requirements, consult each instrument's respective site preparation guide. The site prep guides are not found within the context of this tool so please reach out to your local Agilent representative should you need them. Now I can exit this room and enter another in a few different ways. First, if I click on any of the panes to the left, it will take me directly to that room or back to the lab floor plan where we were originally or I can locate the blue dot on the door where it will take me into the next room.
If I hover over it, I see that this room will be for metals testing. As we enter the room, we find the Agilent 7,850 ICPMS with SPS four Auto Sampler for heavy metals analysis. Again, you will find the blue hotspot on the instrument for a short informational video and one on the monitor for additional resources. In this room, you will notice the rough pump for the ICPMS in addition to some other necessities. In the corner we have an argon dewar to supply the appropriate amount of gas to the ICPMS.
Additionally, as I rotate here you will find a hood and microwave digestion system. A means of digestion is required for heavy metals analysis and while there are various types of digestion systems available, all utilize acid and almost vent directly into a hood. As such, it is a good idea to have a hood in this room. As before, if I hover over this door, I can see our next adventure will be to the residual solvents And terpenes Testing Room. As we enter the residual solvents and terpenes testing Room we find the Agilent Intuvo 9,000 with 5,977 single quadrupole detector and 8,697 headspace sampler for residual solvent analysis.
Next to it, we have the same configuration only we have added a liquid auto sampler to it so that we can also analyze terpenes on the same instrument. Again, we see the rough pumps that are required for both instruments on the floor. Now, in some situations it can be a good idea to have this room under positive pressure. That is because you will be using many organic solvents throughout the laboratory. So as I scan this room to the right, I see both a freezer and a flammable cabinet to store those chemicals and standards.
This time instead of going through the door, I am going to click back on the lab floor plan and enter our last room, the microbial testing room. I am doing it this way so you can notice that the micro and residual solvent rooms are situated next to each other. That is because if you choose to place the residual solvents testing room under positive pressure, which is an absolute requirement for your microbial testing room, then you won't need to run the associated plumbing all over your lab space. So finally, let's take a dive into the microbial testing room. In here you will find all sorts of fun things including the Agilent Aria MX qPCR and Bravo automated liquid handler.
Due to the large differences in solvents for all of the other workflows, such as 50 ml extraction, but a one ml final dilution, automating the sample preparation steps has been notoriously difficult. Here, however, we do have the ability and we have successfully implemented this at many locations. As I scan this room to the left, you will notice a freezer, a large refrigerator, a benchtop incubator, a centrifuge, a microscope, and even some PPE. Some of the items located in this room will depend on the offerings that you choose to provide, so please speak with your Agilent or medicinal genomics representative to discuss. Now, a reward for sticking around.
Throughout this virtual learning lab, we have hidden additional hotspots for you to find. No, we're not going to tell you what they look like nor where they're hidden. Some of them will contain free consumables or discount off your next consumables order. So take a look around the lab, see if you can find them and you might just be one of those lucky winners. The link to access the Agilent Cannabis Virtual Learning Lab will be sent to you in an email after the webinar.
You will also be able to access it via the chat during the live Q and A session directly following Jean Francois Roy's presentation. Now, let me hand this over to Jean Francois Roy to discuss some new Agilent cannabis testing applications.
Hello everyone. My name is Jean Francois Roy. I am an application scientist doing mostly mass spectrometry and I'm based out of Montreal, Canada. Today, I will be talking about recent work that Agen has been doing in the cannabis testing market. And you will see that this work is focused on terpene testing.
But before we start, I would like to remind everyone that Agen products and solutions are intended to be used for cannabis quality control and safety testing in labs where such use is permitted under state and or country law. So, the update work I will be talking about today will be compared to the original terpene testing work that was published a few years ago. The document number for this original application note is 5990Four-2032en and this is still the preferred terpene testing work that Adjunct recommends. However, again, the updated work, if you will, is the fruit of customer requests and discussion with our customers that have been using the original work. So the first app note I will be talking about obviously is a reflection of this where some of you have been asking for automation of sample preparation.
This is exactly what we're showing in this first update app note and the sample preparation was automated using the Agilent PAL three Series two RTC or robotic tool changing sampler. As can be seen on the left hand side of the slide, the RTC rail can be fitted with multiple tools that can be used sequentially by the sampler. On the right hand side, one can see that for the preparation of the calibrance, there's a lot of pipetting and diluting that is involved as well as some vortexing. So the RTC rail and sampler can do that with a program and that can be done obviously without human intervention. So once the program has been written, then the sampler just does the calibrant preparation.
Same thing can be done with the sample preparation. However, the only manual step here is step one where weighing of the dry flour has to be done offline manually, if you will, in a vial and then those vials are loaded onto the sample tray on the sampler and then the rest of the sample preparation can be done by the sampler itself. Benefits for this approach, obviously reducing the error rate that is typically observed in this application. As well, there is a possibility of reducing the starting material and thereby reducing solvent consumption as well if this is something that the regulation that you are working with will allow. And last but not least, human resources are typically required to do the sample preparation can be assigned to other tasks in the lab while the sampler can work unattended 20 fourseven.
The second application note that I will be talking about uses two new features compared to the original work that is hydrogen carrier gas instead of helium and the hydro inert mass spec source compared to the extractor source. So, a word on carrier gas. Helium, although sometimes hard to source and sometimes also very costly, is still the preferred carrier gas option for GC. Agilent does not recommend converting to another gas and there are multiple options out there to evaluate and ultimately to reduce your helium consumption, namely performing helium audit comparing your theoretical helium consumption and compare it to your actual helium consumption and see if there are any major leaks in your pipes and in tubings as well as the gas saver option that will allow to reduce by half helium consumption during a run. Last but not least, the helium conservation module that will allow you to switch from helium to nitrogen while the system is standing by.
So those options are readily available and they will allow you to greatly reduce your consumption of helium. Hydrogen and nitrogen are viable carrier gas alternatives, but they both require special consideration. Namely, hydrogen is flammable, it's reactive and it can also lead to premature failure of consumables. Changing or converting from helium to another carrier gas will result in changes in chromatographic results as well as chromatographic parameters. This is definitely not a plug and play experience.
One direct change that is associated with conversion of helium carrier to hydrogen is that you need to adapt your columns. Because of the physical properties of hydrogen compared to helium, the internal diameter of a GC column when using hydrogen needs to be adapted, needs to be smaller. Typically as well, the stationary phase is also reduced. So, if we have a look at the original work for terpene testing that was published with helium carrier, This work uses two columns at 30 meters and 0.25 millimeter internal diameter and 1.4 micron film thickness. With this work that was published on hydrogen carrier, There's still two columns but they are 20 meters each.
The internal diameter was reduced to 0.18 millimeters and the fill thickness was reduced to one micron. Another change between the original and the updated work is the mass spec source. To make a long story very short, hydrogen is very reactive and can lead to, again, in source reactivity leading to different fragmentation pattern. So Agen came up with this new source called called HydroInert that will enable the exact same fragmentation and source the exact same pattern when using hydrogen compared to helium. So, when combining those aspects together, one can compare the original chromatography that was established with helium carrier in about thirty minutes separating 40 terpenes and showing one internal standard.
With the hydrogen carrier gas on Intuvo, run time was reduced to about twenty one point five minutes without any loss in separation. Finally, the last app note that was recently published is using 8890GC along with hydrogen carrier and hydro inert sources. This is another request that we've had where again, our original work was using Intuvo GC. Some of our customers wish to be able to use the eight thousand eight and ninety so what we've done here is we're just showing that the work can also be done on the 8,890 with hydrogen carrier and although the chromatograms look highly similar, you will see that there are slight differences here in terms of resolution and retention times but all in all, chromatography is again highly similar between the Antuvo GC and the ADA89 GC with hydrogen carrier and hydro energy source. The three update atmos that I talked about today, the work in those atmos was done by my colleagues Sam Harad, Sarab Patel and Jessica Westland.
So I'd like to acknowledge their work and thank them for this. And I will also thank you for your attention and we'll take any question that you may have.
I would like to remind our audience how to submit questions. You can submit questions by typing them in the Q and A box, which we found directly below the video player. Greg, I'll turn it to you to ask the question.
Thank you very much. Thank you everyone for sticking around and listening to this presentation. Like we said, we want this to be interactive. So if you have any questions, please go ahead and type them in. We'll start by answering the ones that have been submitted already.
I know we already answered this one, Heather, but let me ask this question from Netherlands. Good evening. Can you supply the link to this virtual lab? Greetings from Netherlands. Where can they find the link to this virtual lab, Heather?
That should be supplied to you now in, the Q and A portion. You should be seeing that now. In a follow-up to this presentation, there we go, just sent it to everyone. You so you should have that now. You should be able to log on and navigate throughout.
And if you have any questions, you can drop them in. Additionally, you're going to get a follow-up email after this. It'll be located there as well. So for anybody watching this feedback, hopefully, you've already received the email and you'll have access to it there too.
Thank you so much, Heather. Next question here. And I know you mentioned something about this in your presentation too, but, for the audience here, can I find the voltage requirements and the ventilation needs for each instrument in this tool? If not, where could I find this?
Great question. And yes, that was in my presentation earlier, but in case you missed it, every instrument has what we call a site preparation document. And it is a good idea to get your hands on these prior during lab build out so you make sure your contractors have everything that they need and you design accordingly because quite frankly, labs can never have enough power. Those site prep documents are not contained within the context of this tool. You'll need to reach out to your local Agilent representative to acquire them.
Service, as well as sales should have access to them. If you're not sure who that is, you can always send it to the cannabis. Inquiriesagilent dot com email, which will also come in that follow-up email to this webinar should you have any additional questions about that.
Excellent. Thank you so much, Heather. Next question, I believe, will be for Jean Francois. Someone in the audience asks, I noticed that you're recommending helium in some of these application notes. If you still recommend helium as the primary carrier gas, why do you present, applications developed with hydrogen carrier gases?
That's a very good question. Basically, it comes down to the availability of helium gas and also associated with it. So there have been areas where you cannot purchase helium pure enough to be used as a GCMS carrier gas. Also the costs in some areas and some instances have been prohibitive. Hence we've had multiple customer requests asking if it was if using hydrogen carrier was doable and as such we have started working on it.
But as you just mentioned, we still recommend helium as the preferred carrier gas and hydrogen as a note or rule of thumb, hydrogen carrier would need to be tested with every compound in every matrix for every application, before it actually can be the transfer can be effectively done. So this is, again, not a slam dunk whenever you're transferring from helium to hydrogen, it needs to be planned accordingly.
Thank you so much, JF. We have another question here. Not sure it has to do with helium or hydrogen, but, speaking of terpenes, do you have do you experience any tailing or shouldering problems if one terpene is really high? I assume the person is talking about concentration when they mean really high. So JF, do you think you could maybe answer that with some of your experience?
And I'll repeat the question. Do you have any tailing or shouldering problems if one terpene is really high?
We haven't seen that. However, we've been working with a given defined concentration range. So this is definitely a question that would need to be further investigated in terms of the actual concentration of the terpene of the tailing terpene. One thing I'm going to say as well is just like potency when the focus is on the major therapy just like the major cannabinoids, you can use a given or certain dilution factor and if you are interested in the low concentration therapies then you may need to adjust the dilution factor forgetting about the major terpenes, right. So it always comes down to the difference in magnitude in terms of concentration of the terpenes and if someone is, sometimes is doable to analyze them all in one sample, but sometimes you also need to further dilute and do sort of a two injection or dual injection setup.
Dilution is the solution to pollution. Thank you so much, JF. And switching questions now for Heather. We have a question that came in to say, very cool lab layout design. How close to reality is this schematic?
Great question. So as I mentioned, in the presentation, these the instruments were actually taken from their AutoCAD files. So they are very they are supposed to be to scale, right? And we only we made the decision to only address the four kind of rooms or workflows, I would say, that we really operate in and have true expertise in. Right?
We do have expertise in the other areas, but individual preferences, the consultant that you're working with, prior experience will all play into some of those other considerations. So within the context of the four rooms that you can go into, the goal was to make it as to scale and as detailed as possible. If there's any deviations from that, such as not showing the tubing going to a GCMS instrument, for example, that was mentioned in the presentation.
Thank you, Heather. And speaking of the layout tool, another question that came, about this, how did you decide which resources to include in each section?
Another good question. So those are meant to be our current and up to date pieces of information. Our goal is to continue to update this. We debated calling it a resource hub, for example. The naming of this was really something.
So we wanted this to be a a twofold tool. So as you may notice, it has implications and potentially fields outside of cannabis. You know, if I'm just looking to purchase an instrument, then you could see what it looks like. But when it came to the resources, we wanted it to be as up to date as possible, easy to navigate because sometimes going to other vendor web pages, it's difficult to find what you're looking for here. Breaking it down by workflow, we're hopeful that it should be easy to locate and up to date.
So as I mentioned in the presentation, you might want to bookmark it because our goal is to keep this as updated as possible.
Thank you, Heather. Another question came here, but, this time it's a little bit more technical, for JF. And I'll ask the person who wrote this question to maybe rephrase, but I hope I get it right. But I believe the person here is asking about the Intuvo system. For the Intuvo system, what are the connections or supplies you would recommend, that would help with troubleshooting situations?
JF, is there any recommendations that you would have on that? And if I did not get the question right, to the person in the audience, please, write back and re ask us this question if I didn't get it right. So just to reiterate, for Intuvo Systems, JFRGC, what's the connection or supply that you would recommend that will help with troubleshooting situations?
All right. Maybe it's a it will be two step answer. If we're talking about hydrogen then the type of consumables that you will want to use is definitely the ultra inert ones. Hydrogen is obviously very reactive and if you have any active what we call active spots in the sample flow path, it may lead to weird chromatography, severe tailing and or degradation of some compounds. So the ultra inert consumables would be preferred.
If we are talking about Opdivo generally not thinking about specifically hydrogen or helium then my typical troubleshooting recommendation is I mean, would need to know more here for if the customer has a specific issue that they're trying to tackle. However, in terms of the consumables that are required, everything is available on our website. As far as troubleshooting a specific symptom, we need to know more. One recommendation I will make, if you are stuck with a given issue, a troubleshooting issue is to try to simplify the system as much as possible. And an example of this is instead of tracking 40 terpenes, just go in a very simple one compound setup short column and see if the problem is still observed, right.
So you will know right away that if it's a compound dependent or it's system dependent. That's what I mean by splitting the system in half and keeping things simple. Once you have identified the source, the root cause of the issue, then try to see if it was just a faulty part that needed to be replaced or it's dependent on the application and the fault keeps coming back in which case we need to address this issue and see if it's a sample prep issue or a consumable that is not meant to be used in this specific application context. I hope this answers the question.
Yeah. I assume it would be on a case by case basis to JF. So, definitely reach out to us, a member in the audience to help you with your specific situation. So, one more question here when it comes to the lab layout design. Someone in the audience asked and I think this question goes for you, Heather.
I have additional questions about lab layout design. Who can I talk to about this?
Yep, great question. Go ahead and you can reach out. If you don't know who your Agilent rep is, I know I've said this a few times already, you can send it to the cannabis. Inquiriesagilent dot com email. Greg, who's actually talking right now, monitors that email and I'm sure he promises to pick up your email and send it to the right hand.
So, go ahead and send it to that email note and we'll make sure that the right person contacts you to discuss that further. Additionally, we had a previous webinar, I believe it was in February to discuss more specifically the lab layout and considerations for a layout such as you know what goes in each room, where we do go a little bit more detailed there. That wasn't the goal today. Today was to introduce this virtual lab. If you would like, I believe that the link to that previous webinar should be sent out or we can send that to you.
We'll be sending it to you, think, if you haven't if you want to hear more about that. If you reach out to the cannabis inquiries email, we can send you a link to that previous webinar we did in February that goes into much more detail to discuss the lab layout.
Thank you, Heather. And I believe also it's in the resources section, here. If you need that, So feel free choose, either option, to contact us. Now one last question that came in about, hydrogen carrier gas. And JF, you might have touched on this a little bit more, but thinking ahead with new methods or new ways to reduce cost, are you working on developing other cannabis testing applications with hydrogen carrier gas?
Yes, we are. There's nothing officially published yet, but we are working on a residual solvent application note that will use hydrogen carrier gas. There's also an effort going on right now to better understand if hydrogen carrier can be used for pesticide testing. So again, we know that hydrogen carrier typically gives a bit less sensitivity when compared to helium. So obviously in the pesticide testing arena, we need the limits or the action limits are typically very low.
So we are currently trying to see if hydrogen can be used for as a carrier gas for hydrate for pesticide testing in cannabis.
Thank you so much, JF. So please stay on the lookout for that. I do have another question that just came in and this is very pertinent and apropos with what's going on recently with recent research papers. So a person in the audience asks here, what system analytical method would you suggest for speciation of thiols, mercaptans sulfides? These are important but understudied tested compounds in cannabis and I believe a recent study was out about this about how two strains with the identical terpene compounds, terpene profile had actually different aromas and tastes.
And I think these are the next class of compounds that are defining aromas. So, let me re ask that question there, JF. I think it's for you, but Heather, free to jump in if you have anything to add to. The question was what system analytical method would you suggest for speciation of thiols, mercaptans, sulfites? These are important but understudied tested compounds in cannabis.
That's a great question. As a rule of thumb, if you have to choose an application to migrate or to go from helium to hydrogen, you'd like ideally to pick compounds that are stable unreactive and what we've got there tiles, mercathen sulfides will react readily. So as far as the recommendation is concerned, I would definitely go with helium carrier versus hydrogen and to better understand them, classify them see from a chromatographic standpoint where they elute compared to the terpenes and the cannabinoids and from then make an educated decision on how if hydrogen can be used as carrier gas. But for sure, would start with helium with those compounds because in my opinion, they are very, very reactive and I would yes, hydrogen will be a challenge with those for sure.
Thank you, Jeff. I think that's about it for the questions that came in. So, Megan, I think I'll turn it over to you.
Wonderful. Thank you so much, Greg. So with that, we will be wrapping up today's event. I want to thank the audience for participating and attending today's event, and I would also love to thank our speakers for the wonderful amount of information they've given us today. I would also like to thank personally our sponsor Agilent for making today's educational webcast possible.
We would like to ask everyone in the audience to participate in a brief survey. This survey will appear on your screen after today's presentation has ended. You'll receive an e mail alerting you and this webcast will be available for replay. We invite you to forward that announcement to your colleagues who may have missed today's live event. Thank you so much to everyone for joining.
We'll see you next time. Have a great rest of your day.