I'll begin by providing an overview of our operational performance, followed by a review by Tracy of our financial performance during the three months and six months ended June 30th, 2022. Following that, we will open the line to take your questions. The close of the second quarter marks a little over a full year of operations for iSpecimen as a public company. At this time last year, the world and our expectations of what was to come certainly looked drastically different. Yet in many respects today, the outlook remains remarkably similar. Geopolitical unrest, ongoing COVID concerns, and a difficult economic environment have reduced near-term visibility for our business. Fortunately, demand for a centralized marketplace to address the inefficiencies of the fragmented biospecimen supply chain remains strong and validates our continued investment in the business. Against this backdrop, we remain intensely focused on fortifying and extending the iSpecimen Marketplace platform.
Specifically, we've been expanding its capabilities to better support our research clients' needs for sourcing critical biospecimens for medical research, along with making it easier for our growing network of healthcare providers and biospecimen suppliers to offer their samples and engage with the platform. As discussed during our previous earnings call, in the second quarter of 2022, we introduced the first of a series of updates to the iSpecimen Marketplace, each aimed at delivering incremental yet foundational improvements to our software platform that align with our long-term strategy. We designed the marketplace search capabilities to make it even easier for researchers to seamlessly identify biospecimens that match their requirements. We also enhanced the platform with new data integration capabilities that simplify the ingestion and maintenance of biospecimen inventories on our platform. To help guide our development efforts, we named Evan Cox to Head of Product in the second quarter.
Evan brings decades of healthcare data experience, having served in a variety of product management leadership roles over the course of his career. While he has only been on board for a little more than a quarter, Evan and his team are prioritizing marketplace capabilities that are expected to increase user engagement with the platform and drive revenue growth. His current focus is on the development of a unified search experience for our customers and our suppliers, which will support the search for both biospecimens and research subjects from whom biospecimens may be collected prospectively. This capability will help bring higher volume and increased velocity to our sales pipeline, specifically for the prospective collection part of our business, which comprised about 25% of our revenue in the second quarter.
As part of this process, we have both strengthened our internal technology development team and have hired outsourced software development teams to accelerate our time to market with these features. Our P&L going forward will reflect an increased investment in our technology development efforts. Concurrent with our work to enhance our software platform, we have continued to expand our global biospecimen supplier list with the addition of new suppliers that specialize in oncology and cardiovascular disease research. These suppliers and their biospecimen samples are now accessible via the iSpecimen Marketplace platform. It is important to stress the significance of the company's efforts to expand and diversify our roster list of healthcare providers. The supply of biospecimens derived from healthcare providers, including biofluids, solid tissue, and hematopoietic cells, is what fuels medical research and its various applications such as biomarker identification, biomarker validation, diagnostic test development, and drug discovery/development.
By ensuring the iSpecimen Marketplace remains the premier platform for suppliers of biospecimens, we will continue to be the go-to platform for researchers who need them. On the topic of researchers, we continue to make investments in the growth of our customer-facing sales team. In the first quarter, we made a strategic decision to change our sales team structure and territory coverage strategy, overall to improve productivity and efficiency going forward. Specifically, we moved to a sales pod structure where junior account executives are paired with senior salespeople, along with marketing and operations personnel who all work together as a unified multidisciplinary team. This pod structure has been designed to move more opportunities through our sales funnel. However, the biggest benefit of this structure is that it frees up our senior sales staff to focus on deeper account management, repeat business, and on closing larger deals.
These efforts are just now starting to pay dividends, as evidenced by the recent closing of a $1.9 billion purchase order in late June and an overall return to growth throughout the early stages of our sales funnel. Next, I'd like to touch on the impact that the conflict in the Ukraine continues to have on our business. As we discussed during the Q1 call, the war in Ukraine disrupted a number of our top suppliers in both Russia and Ukraine. The good news is that many of the Ukrainian sites are now either back or coming back online and offering favorable economics to iSpecimen. The challenge is that they currently have limited capacity and that considerable uncertainty in the region remains. To minimize our risk, we are using our Ukrainian sites cautiously and only on projects where additional suppliers exist.
We expect the reduction in the use of these sites will continue to have a modest impact on both the overall value of our purchase orders and on specimen fulfillment velocity against those purchase orders. Additionally, I want to address the overall economic environment and its effect on our business. Our customers are primarily companies involved in the development and commercialization of diagnostics and or therapeutics, and many of them are small to mid-cap companies that rely on regular infusions of capital to fund their research and development pipelines. Since early 2022, access to new capital in the biotech/life sciences sectors has plummeted along with company valuations. We believe this has caused many companies to be more cost conscious in an effort to conserve cash until the markets recover.
While it's too early to know the extent to which our sales funnel has been impacted by these challenges, we have experienced a reduction in the average size of purchase orders by about 20% over the past six months, meaning we are fielding smaller projects overall, which we believe is a function of the economic slowdown. On the positive side, we have also seen a corresponding increase in the overall number of purchase orders, which has kept our purchase order value relatively constant in the first half of this year compared to the first half of 2021. I'm confident that the changes we've made to the sales team structure, i.e. the pods, will help reverse this trend by allowing our top salespeople to focus more of their time on account management, larger projects and purchase order growth.
Finally, it wouldn't be a typical earnings call if I didn't say something about COVID. While COVID specimens now represent a small part of our revenue today, we continue to pay close attention to what a new outbreak could mean for our customers and our suppliers. While we have a geographically diverse supply network and believe that we can maneuver around localized COVID outbreaks, the overall economic disruption caused by COVID may still affect us in the future. That said, at this time, we believe that COVID is not dramatically impacting our business and our ability to source specimens. Despite these challenges during the first half of 2022, we remain confident that the execution of our strategy, along with our investments in technology and key personnel, especially the sales and marketing teams, will continue to improve our position to gain additional market share over the coming quarters.
I also believe that our entire leadership team has done and continues to do a great job navigating the challenges of the past two years. We all look forward to realizing the benefits of ongoing development work and the restructuring of our teams and returning to growth. Now I'll pass the call on to Tracy Curley, who will review our financial results. Tracy?
Thank you, Chris. Good morning, everyone. Today, I'll review our financial results for the three and six-month period ended June 30, 2022, compared to the same period in 2021. We reported revenue of $2.3 million for the second quarter of 2022, compared to $2.9 million for the second quarter of 2021. The decreases in revenue for the three-month period in 2022 were primarily attributable to the continuing decline in sales of COVID-19 specimens when compared to the same period in the prior year. During the six-month period ended June 30, 2022, we reported revenue of approximately $4.9 million compared to approximately $5.9 million during the same period last year.
The decreases in revenue for the six-month period in 2022 were primarily attributable to the impact of the Russia and Ukraine war in the first quarter of 2022, which shut down our supply sites in those regions and impacted our ability to fulfill orders at the start of the war, and a continued reduction in sales of COVID-19 specimens when compared to the same period in the prior year. We anticipate that our COVID-19 revenues will continue to decline, but do not have specific guidance on the decline due to the uncertainty of the continued impact of COVID-19 that Chris just spoke about.
For the three months ended June 30th, 2022 and 2021, revenue derived from specimens related to COVID-19 accounted for approximately $170,000 and $896,000, or 7% and 31%, respectively, of our total revenue. For the three months ended June 30, 2022 and 2021, revenue derived from non-COVID-19-related specimens accounted for approximately $2.2 million and $2 million, or 93% and 69%, respectively, of our total revenue, an 8% increase in non-COVID-19 revenue compared to the same prior year's period. Specimens accessioned during the three months ended June 30th, 2022 increased by 738 specimens, or 12%, - 7,004 specimens, compared to 6,266 specimens accessioned during the three months ended June 30th, 2021.
However, there was a change in specimen mix that resulted in a decrease in the average selling price per specimen of approximately $130, or 28%, compared to the same prior year's period. For the six months ended June 30th, 2022 and 2021, our revenue derived from specimens related to COVID-19 accounted for approximately $649,000 and $1.6 million, or 13% and 27%, respectively, of our total revenue. Specimens accessioned during the first half of 2022 increased by approximately 544 specimens, or 5%, to approximately 11,928 specimens, compared to approximately 11,384 specimens accessioned during the first half of 2021.
However, a change in specimen mix resulted in a decrease in average selling price per specimen of approximately $132, or 26% compared to the same prior year's period. Cost of revenue decreased by 33% from approximately $1.5 million for the second quarter of 2021 to approximately $1 million for the second quarter of 2022. Cost of revenue for the six-month period ended June 30th, 2022 was approximately $2.2 million compared to approximately $3.1 million for the same period in 2021, a decrease of 30%.
The second quarter of 2022 decrease was attributable to a 40% decline in the average cost per specimen impacted by specimen mix, offset somewhat by a 12% increase in the number of specimens accessioned for the current period compared to the same prior year's period. The six-month period ended June 30th, 2022. Decrease was attributable to a 44% decline in the average cost per specimen impacted by the specimen mix, offset somewhat by a 5% increase in the number of specimens accessioned during the six months ended June 30, 2022 over the same prior year's period. For the second quarter of 2022, we increased our cash spend for technology to approximately $807,000 from $351,000 for the same prior year's period.
For the six-month period ended June thirtieth, 2022, we increased our cash spend for technology to approximately $1.4 million from $740,000 for the same prior year's period. The increase in spend for the three- and six-month periods ended June thirtieth, 2022 compared to the same prior year's periods is directly related to our commitment to investment in our technology, as evidenced by our recent launch of our enhanced iSpecimen Marketplace platform. For the second quarter of 2022, this cash outlay was comprised of approximately $438,000 of capitalized internally developed software and approximately $369,000 of technology expenses that we were not able to capitalize and therefore were classified as technology expense.
The remainder of the technology expense for the second quarter of 2022 was comprised of approximately $267,000 of non-cash amortization related to internally developed software. Total technology expense for the second quarter of 2022 was approximately $636,000. For the six-month period ended June 30th, 2022, this cash outlay was comprised of approximately $777,000 of capitalized internally developed software and approximately $630,000 of technology expenses that we were not able to capitalize and therefore were classified as technology expense. The remainder of the technology expense for the six-month period ended June 30, 2022, was comprised of approximately $533,000 of non-cash amortization related to internally developed software.
Total technology expense for the six-month period ended June 30, 2022 was approximately $1.2 million compared to $772,000 for the same prior year's period. Sales and marketing expenses were approximately $951,000 for the second quarter of 2022, compared to approximately $648,000 for the second quarter of 2021. For the six-month period ended June 30th, 2022, sales and marketing expenses were approximately $1.7 million compared to approximately $1.2 million during the same prior year's period. The increase was attributable to increases in payroll and related expenses, external marketing efforts, and general operating expenses.
General and administrative expenses were approximately $1.6 million for the second quarter of 2022, compared to approximately $1.5 million for the second quarter of 2021. For the six-month period ended June 30th, 2022, general and administrative expenses were approximately $3.4 million compared to approximately $2.5 million during the same prior year's period. The increases were primarily attributable to an increase in costs related to compensation, non-cash stock compensation, directors and officers insurance, and other operating and maintenance expenses. As of June 30th, 2022, our cash balance was approximately $23.7 million compared to approximately $27.7 million as of December 31st, 2021. This concludes our prepared remarks. Now I would like to open the call for questions. Operator, please go ahead.
Thank you. At this time, we'll be conducting a question-and-answer session. If you'd like to ask a question, please press star one on your telephone keypad. A confirmation tone will indicate your line is in the question queue. You may press star two if you'd like to remove your question from the queue. For participants using speaker equipment, it may be necessary to pick up your handset before pressing the star keys. Our first question comes from the line of Matt Hewitt with Craig-Hallum Capital Group. Please proceed with your question.
Good morning, and thanks for the update and for taking the questions.
Pleasure.
Maybe first up, you mentioned that you continue to plan on incremental investment on the tech side. I'm just curious, as you look out over the next, you know, year or so, what are some of the areas that you expect to enhance on the platform?
Yeah, this is Jill Mullan, I'll take that. We're really looking at how do we integrate what we call unified search, which is bringing the search for biospecimens as well as the search for patients together in one interface. Today, if you go to our marketplace, you can search for specimens that exist in biobanks or clinical labs. If you wanna do a prospective collection from a patient, you basically tell us what you're looking for, and we go out into our network to see if they have it. The unified search will allow users to go in and type in their criteria or use the checkbox and sliders to enter their criteria and get a view immediately into what's available at our network in terms of patient encounters or likely patient encounters. That's the big feature we're working on.
It requires doing some work on the database to integrate it all together and then to make those capabilities available to the researchers.
Just to add, Matt, to what Jill just said, the unified part of unified search is that over the years of us evolving our technology here inside the company, it's evolved in silos where we have had one set of technology illuminating access to clinical remnant specimens, for example, in clinical laboratories, and another technology, another silo that would look at inventories of biobanks. It's time for us to now put that onto a single platform so that we can allow a customer to search in all those environments with the same sort of uniform experience.
Including the patients.
Including patients.
That's great. Thank you for the color there. Maybe regarding your commentary regarding obviously the geopolitical headwinds, that was. It sounds like it was more first quarter versus second quarter, but I'm assuming they're still there. And then regarding the economic impact, thank you for the details that you know, you're seeing on the smaller customer side. I'm wondering if there's a way to kind of elaborate onto, I guess, one, what is there a way to quantify maybe what those headwinds are from a dollar perspective or from an order size perspective? And then secondly, as you look out at the back half of the year into next year, do you know.
How do you see those headwinds maybe subsiding or could they at some point create this backlog of orders that she would expect to see?
We're not giving guidance on that. We dealt swiftly in Q1 when the war started with existing purchase orders that we had anticipated being filled by our supplier sites in the Ukraine or Russia. Even though our Ukraine sites are coming back online, we are very cautious because as you know, the war is still going on. We don't have any hard numbers, and we certainly are not sole sourcing a PO in the Ukraine like we did in the past. There are things that we're doing that are more addressing the risk associated with that. Jill, do you wanna add any more to that?
Yeah. I think we said in the first quarter that we had about $1 million worth of purchase orders that were affected at that time. That doesn't mean it was all revenue that was expected in the first quarter 'cause some of those purchase orders collect over multiple periods. As we entered 2Q with the Ukrainian sites still either you know slowed down or not operational, what that did is it impacts feasibility assessments and the ability to send orders their way. That's why we say there's some lingering effect there. At the same time, we've been looking to like how quickly can we augment it with other sites that have equally good economics and are over in those areas of Europe to be able to support the same sort of projects.
We've added sites in, let's see, Turkey, Armenia, Estonia. We're looking to augment those sites so that we can continue to, like I said, get the same economics in the same region.
That's very helpful. Thank you. Then maybe one last one for me, and I'll hop back into queue. Obviously a strong performance in the quarter on the gross margin side. You went through some of the details there. Just how should we be thinking about that over the back half of the year? You know, is that 57% sustainable or is it completely dependent upon mix and so it's hard to kinda point to a range even? Thank you.
We generally are shooting for, you know, margins that are in the 50%-60% range. This quarter came in better than that in large part because of specific projects and their mixes. I think going forward you'll see it, you know, pretty consistent with how it's been. It may be lumpiness based upon projects.
I would like to say you might remember last Q3 we did discuss the fact that we had modified the way we were calculating our cost of revenue because our reporting had gotten more precise internally, and we were able to get more accurate with that. Part of that lower percentage for cost of revenue to revenue ratio is a direct result of that. You'll continue to see that as well.
That's helpful. Thank you very much.
You're welcome.
Thanks, Matt.
Thank you. Our next question comes from the line of Constantine Davides with EF Hutton. Please proceed with your question.
Thanks. Chris, the large purchase order you called out, I was wondering if you could just expand a bit on that. What type of company was it? Was it a new or existing customer? I guess what enabled you to identify and ultimately capture an opportunity like that?
I'll let Jill give some of the details, but I'll answer part of that, Constantine, that it is a little bit of both. It's a new customer for us in terms of it being a new entity buying from us. As you and all the others on the call already know that there's people rotate through different life science companies, and it's an audience that you may serve them in one company, and they may reappear in another company. We've done a good job of maintaining those relationships. I say that they're somewhat of an old customer because we've worked with these folks before and have done well in their prior companies, and they have followed us with this new company and this new project.
I'll let Jill speak more to the details of what it is to the extent she can.
Yes, I can say that it's a prospective collection, and it's oncology-related, which is pretty typical for those prospective collections. It's a long-term project, so it'll be collecting over the course of the next year plus. I think one of the big reasons that we want it is that it's very data intensive and it's a hard project to do, but because of our technology and our data team and the data capabilities that we have, we're able to handle it.
It does exemplify, Constantine, what we're trying to harvest more of with the pod structure that we're going to free up the senior salespeople to be able to engage in a more consultative fashion with teams to be able to get these larger, longer-standing projects. We're hopeful that this is the first of many more to come.
Great. And then just, I think you said in your comments, Chris, 25% of the revenue was prospective in the quarter. How does that compare sort of year-over-year? And second, I understand, you know, there's different projects that may hit in a given quarter, but just on average, how much of a pricing and maybe margin differential is there between prospective and I guess more sort of classically, you know, remnant-oriented volumes?
Yeah.
Let me start with the pricing question first. Prospective collections typically are priced at $1,000 on up, and it can be $2,000, depending upon how complicated the project is and the amount of data that's required.
Per specimen.
Per specimen or per collection. On the remnant side, which a clinical remnant is in the tens of dollars per sample, so, you know, $50-ish on up. And then you have in between, tissue blocks and archive samples that are typically coming out of pathology or a biobank, and those specimens are in the mid- to upper hundreds. Prospective collections tend to be much more valuable than the clinical remnants, obviously. I think the second part of your question was around the mix of prospective versus other projects. In a typical quarter, or if you look at the average across a whole bunch of quarters, we're typically 10% ±5% on remnants, so between 5% and 15%.
The rest is typically split fairly evenly between prospective collections and banked projects, which means we're typically somewhere around 40%-45%, up to 50% prospective as well as banked, so depending upon how much we have in terms of remnants. This quarter, the 25% was low. That I think has impacted the ASPs, and I think Tracy went through them, and they were a little bit lower than they typically are, and it's because we lost that high value part of our mix, or at least we didn't have as much of a high value part of our mix. This big purchase order that we just took, as I mentioned, was all prospective, and we expect to see that mix shift back to something that is more typical.
Is there much of a margin differential, or does it really just depend on the project?
It depends upon the project. There's not typically a margin differential across our various segments that we look at. They all tend to be in the same ballpark.
Got it. Okay. Thank you.
Welcome.
Thanks.
Thank you. Our next question comes from the line of James Liberman with Revere Securities. Please proceed with your question.
Great. Thank you. It's terrific to see the progress you're making in your unified search platform and the increase in customer organizations purchasing from you up 21% and the registered research and user group up to 32%. It sounds like your reach is expanding. I was also impressed by the $1.9 million order. That's a very good sign. I wonder if whether you can comment. Do you see this reaching some sort of a tipping point where you know suddenly it's this aha moment out there? Obviously, it's not a magical thing. You've got to do a lot of contact. Is that something that could happen, where you see like multiple multi-million-dollar type agreements like you've just signed?
Thank you for the comments and the question, Jim. We're probably all gonna chip into the answer here. I'm gonna say we do envision it a tipping point. The image that's in my mind is we have all of these efforts underway from restructuring and fine-tuning the processes of our sales and marketing teams, investing heavily in Evan Cox leading product management, platform product management for us and the tech team as well. There's gonna be a convergence of capabilities that come out, hopefully in sync with a recovery in the life science markets and some more buying activity.
I do think that we do get to that tipping point, and that's really what's driving what we're doing all these things for, is to drive it towards that convergence where we can be more consultative, and demonstrate to potential customers we have the ability to do these large projects, far better than any of our competitors, given the tech platform and, the way that we go about sourcing from our network. I'll let Jill and Tracy add to that as well.
I think you covered it pretty well.
I don't wanna be the Debbie Downer here, but obviously all of these initiatives do cost money, and so you probably have noticed that our expenses are a little bit up in Q2 this year compared to Q2 last year. We are managing our expenses very closely and carefully as we are progressing in these initiatives that do increase costs.
Yep. Thank you very much for those answers. I feel that the way in which you've structured and prepared and your company and the kind of outreach that you're doing and the resources that you have certainly puts you in a very strong competitive position, and I'm excited about the upcoming calls that you're gonna be having. Thank you.
Oh.
Thank you.
Thank you, Jim. We're all confident. We never anticipated this to be a fast build. It's a difficult market and a different, difficult business model that we've embarked on to do what we do in the absence of creating large inventories like the commercial biobanks with which we compete. But we're all confident that there is a market there and that we have a solution. We hear it from our customers all the time that resonates with them, and that we'll get to that tipping point. Thank you.
Thank you. Ladies and gentlemen, there are no further questions in the queue. At this time, I'll turn the call over to Mr. Christopher Ianelli, CEO and President, for closing remarks.
All right. Thank you, operator. I'd like to thank everybody again for joining us on today's call and for your continued interest in iSpecimen and the good questions that we got at the end of our remarks. We look forward to having follow-up conversations with many of you and seeing you at upcoming events. Please do reach out to any of us, or to the team at KCSA that represents us for investor relations. With that, we'll wrap it up. Everyone have a great day. Thank you.
Thank you.
Thank you.
Thank you. This concludes today's conference. You may disconnect your lines at this time. Thank you for your participation.