Ladies and gentlemen, thank you for standing by and welcome to the Maravai LifeSciences second quarter 2021 earnings conference call. At this time, all participants are in a listen-only mode. Later, we will conduct a question and answer session, and if you would like to ask a question during that time, simply press star one on your telephone keypad. If anyone should require assistance during the conference, please press star zero. I would now like to turn the conference over to Deb Hart, Head of Investor Relations. Please go ahead.
Thank you, Alexander. Good afternoon, everyone. Thanks for joining us on our second quarter 2021 earnings call. Our press release and the slides that accompany today's call are posted on our website and are available at investors.maravai.com under financial information quarterly results. On today's call, we will cover our financial results and the business highlights. We'll provide updated financial guidance for 2021. As you can see on slide two, Carl will first provide you with a business update. Kevin will review our financial results and guidance. We will open the call for questions following the prepared remarks. On slide three, we remind you the forward-looking statements that we make during this call, including those regarding our business goals and expectations for the financial performance of the company, are subject to risks and uncertainties that may cause actual events or results to differ.
Additional information concerning these risk factors is included in the press release we issued earlier today, as well as those that are more fully described in our various filings with the SEC. Today's comments reflect our current views, which could change as a result of new information, future events, or other factors, and the company does not obligate or commit itself to update these forward-looking statements except as required by law. During this call, we will be using non-GAAP measurements of certain of our results and in providing guidance. Reconciliations of GAAP to non-GAAP financial measures are included in the press release that we issued this afternoon, which is posted to Maravai's website and at www.sec.gov via EDGAR. The metrics we will be discussing in today's call include net income, adjusted EBITDA, income tax expense, and adjusted earnings per share.
These adjusted financial measures should not be viewed as an alternative to GAAP measures, but are intended to better enable investors to benchmark our current results against historical performance and the performance of our peers. Now I'll turn the call over to Carl.
Well, thank you, Deb, and good afternoon, everyone. We appreciate having you join us for our call today. Let's start with our second quarter results on Slide five. Maravai had a very strong second quarter, in fact, the largest and most profitable quarter in our history by far. Today, we reported $217.8 million in revenue, growing 364% compared to the prior year, and up 47% sequentially over quarter one. Our adjusted EBITDA of $164.7 million grew 841% over the prior year and 62% sequentially. Our top-line performance and outstanding adjusted EBITDA resulted in adjusted EPS of $0.44 per share and record free cash flow generation. We've seen an incredible first half in our first full calendar year as a public company. It is clear that momentum continues to build across our global customer base as mRNA research and development moves to the forefront of modern medicine.
Turning now to slide six, growth in our Nucleic Acid Production business, in particular, remains very robust. Nucleic Acid Production had record revenue of $192.5 million, up 533% year-over-year and up 55% sequentially. Demand for CleanCap mRNA continues to accelerate in all areas. CleanCap reagents themselves, GMP manufacturing services, and custom mRNA constructs. Full regulatory approval by the FDA of the first mRNA COVID-19 vaccine currently being used under emergency use authorization is now expected to occur around Labor Day and should further expand demand for the vaccine and CleanCap in late 2021 and beyond. Pfizer and BioNTech just announced that they've already shipped over 1 billion doses of COVID-19 vaccines. All of those contain CleanCap. Pfizer has further said that they plan to increase COVID-19 vaccine production by one-third in 2022, going from 3 billion doses this year to 4 billion doses next year.
In addition, the development of new generations of COVID-19 vaccines continues apace. These next-gen vaccines either offer specific protection against emerging virus variants such as the Delta variant, or they enhance overall immune responses by offering booster doses of the first-generation vaccine formulations. On the public health front, we are beginning to see nations such as Israel, France, and Germany offer third doses of mRNA vaccines as a booster to older or immunocompromised citizens. Others, such as the U.K., are additionally recommending that recipients of single-dose vaccines from J&J and AstraZeneca receive an mRNA dose at their next immunization. It also seems that each week we learn of new investments being made in mRNA technology as it represents the future of vaccine development efforts due to its flexibility, scalability, and effectiveness. Slide seven shows some of these headlines.
Sanofi announced a $477 million annual investment through 2025 to accelerate the clinical development of their entire mRNA portfolio. Sanofi has stated that they hope to have a minimum of six mRNA candidates in the clinic by 2025. Last week, they announced their intention to acquire Translate Bio for $3.2 billion to accelerate vaccine programs and to further explore other therapeutic areas for mRNA. They specifically cited a goal to unlock the potential of mRNA in other strategic areas such as immunology, oncology, and rare diseases, in addition to vaccines. This comes on top of the previously announced commitment made by Pfizer to increase R&D spending in 2021 by $600 million for additional mRNA-based development programs. GlaxoSmithKline said that they now have over 200 scientists working on mRNA and are making large-scale investments in mRNA manufacturing. BioNTech recently announced their new project to develop an mRNA-based malaria vaccine.
Their goal is to start a clinical trial by the end of 2022. BioNTech is already collaborating with the Bill & Melinda Gates Foundation on HIV and tuberculosis programs. For TB, BioNTech plans to begin clinical trials for testing a vaccine candidate also in 2022. Currently, they and their partners are developing vaccines against nine different infectious diseases, and the company continues to pursue 15 oncology programs in the clinical and pre-clinical phases based on four different drug classes, including mRNA. Novartis has also indicated that they are considering entering the mRNA market. Chairman Jörg Reinhardt commented in a recent interview that, quote, "The mRNA technology has proven to be an attractive option in this situation. And of course, every research company is questioning whether they should invest more in this area." Close quote.
Celltrion, a leading pharmaceutical company in Korea, announced their plans to develop a next-generation mRNA vaccine platform. We are currently seeing the impact of all of this increased enthusiasm and investment on our own business. To illustrate this point, let me share with you some CleanCap supply agreement statistics. At the start of 2021, we had executed five supply agreements for CleanCap in place and another handful of agreements under discussion. Today, we have 13 supply agreements signed, 12 more in active late-stage negotiations, and 25 more with term sheets under review. This underscores the incredible enthusiasm that exists for mRNA generally and CleanCap specifically. Even more encouraging is the fact that this customer population spans the entire spectrum from large pharma to innovative biotechs to new and potentially transformative manufacturing platforms.
From our perspective, mRNA and CleanCap are clearly here to stay in a durable and meaningful way. Maravai is right in the middle of it. We are also increasing our investment in mRNA innovation as we scale our R&D operations, facilities, and quality systems. We believe that an appropriate level of investment in R&D long term will approximate 5% of our revenue. We would expect to reach that level of investment within the next three years. That investment will be comprised of people, laboratory facilities and equipment, program management resources, and external collaborations. We will also begin pursuing specific technologies and new opportunities identified by our new Scientific Advisory Board, which we are currently in the process of forming. We'll have more information on the SAB available for you during our next earnings call.
Turning to slide eight in our Biologics Safety Testing business, which supports high growth markets in cell and gene therapy, vaccines, and biologics drug manufacturing. Here we set the gold standard in host cell protein and process-related impurity analytics, along with offering innovative viral clearance solutions that ensure the safety of biopharmaceutical products. Our second quarter revenue of $18.2 million in BST marks a record high, up 47% from last year and up 3% sequentially. This growth was driven by three main factors. First, continued high end user demand for our products through both direct and distributor channels as a result of expanding COVID-19 and Adeno-Associated Virus, or AAV, vaccine and therapeutic programs and their unique analytical needs. Second, strong sales across the full breadth of our product line that are routinely used in a number of commercialized cell and gene therapies, oncolytic vaccines, innovative biologics, and biosimilars.
Finally, the continuously expanding biopharma product development pipeline. We saw strong demand for all categories of kits during the quarter, from generic host cell protein assays to other ELISA and purity detection kits, and to orthogonal mass spectrometry-based services that promote the use of our HCP kits. A number of clients entered into agreements with us to develop custom host cell protein assays to support their proprietary biologics. We plan to continuously innovate and scale our offerings in our Biologics Safety Testing business to ensure superior technical support, to offer the highest quality services and products, and to offer the most comprehensive catalog of products to meet our customer needs. Turning now to slide nine. Our Protein Detection business saw 71% revenue growth versus the prior year, which were the pandemic-impacted revenues. Sequentially, revenue was up 6%, driven both by our custom and catalog business.
Vector now represents only 3% of our revenue and 2% of our EBITDA. Our current assessment is that there are multiple significant market opportunities in our Nucleic Acid Production and Biologics Safety Testing businesses that will require incremental investments and management attention in order to support our hyper-growth in these strategically important markets for Maravai. As you will see on slide 10, we announced earlier today that we have entered into an agreement with Thompson Street Capital Partners to sell Vector Laboratories for a purchase price of $124 million in cash. Vector operates in a different market with a different growth profile, that being research immunohistochemistry versus cell and gene therapy than the rest of Maravai, and focuses on different customers, primarily traditional academia versus biopharma.
While we have seen a good recovery in the protein detection business from the pandemic lows, our view is that Vector will require material investments in commercial capabilities and a commitment to bolt-on M&A in order to ensure its long-term competitiveness in this pure research segment. The combined effect of such investments would be to increase our financial exposure to a non-core segment. We prefer to maintain a laser-like focus on Nucleic Acid Production and Biologics Safety Testing as we see those markets being significantly higher growth and far more strategic for Maravai. We wish Lisa Sellers and the entire Vector team nothing but tremendous success going forward. We're confident that Thompson Street is a great partner for Vector and that this divestiture will allow Maravai to focus on our core technologies in cell and gene therapy while supporting our biopharma customer base.
As you might imagine, we are actively evaluating additional acquisition opportunities across the entire cell and gene therapy space. Proceeds from this transaction will further improve our cash position and enhance our ability to execute quickly on meaningful acquisition targets. I'd now like to turn to slide 11 and give you a quick update on our facilities and our plans for additional capacity expansion to support our exceptional revenue and profit growth. As you will have seen, we just entered into a lease agreement to develop a new expanded San Diego facility for the Nucleic Acid Production business. We refer to this as the Flanders site. Our current Wateridge facility in San Diego is already close to reaching capacity in certain areas.
Although we have yet to maximize the total output of Wateridge as measured in sales dollars, we will soon be in need of additional space for offices, warehouse receiving and storage, and for certain laboratory operations. Our objective then is to create a center of excellence where our existing Wateridge facility will continue to be the manufacturing center of excellence for all mRNA technologies. By moving some of our operations to the new Flanders site, we will further increase capacity for commercial CleanCap production, expand the rest of our small molecule platform, enhance our research and development capabilities, and add GMP API manufacturing capacity.
The Flanders site will house two centers of excellence for us, one for innovation and one for oligonucleotides and chemistry that will enable the R&D expansion for chemistry and small molecules and mRNA technology that I spoke about earlier. Similarly, we've entered into a new lease for our Biologics Safety Testing business in North Carolina. Groundbreaking for the new site is scheduled for late 2021, and we anticipate occupancy in Q3 of 2022. The state-of-the-art facility will more than double our operational square footage, supporting current and future growth. The fully customized design will provide room for a mass spectrometry center of excellence and specialized cell culture facilities. It will significantly increase our cold storage capacity while providing other R&D, laboratory, and automation upgrades. Extensive process flow analysis has been incorporated in the design of the facility to optimize and enhance both our manufacturing and kit packaging operations.
Not only are we extremely excited about the additional capacity these new facilities will provide for Maravai, we are pleased with the continued success of our employee recruitment efforts. We ended the second quarter of 2021 with over 500 employees, 507 to be exact, for the first time in our history. To put that in perspective, we have increased our workforce organically by nearly 40% in the last 12 months. I'm extremely proud of our human resources team and all of our supervisors and leaders for their commitment to achieving amazing financial results while remaining dedicated to advancing the human capital capabilities of Maravai during these unique times. Now, moving to slide 12, I'd like to ask Kevin to cover our second quarter and first half performance, the P&L impact of the Vector transaction, and the capital expectations for our facility expansion program, along with our updated guidance for 2021.
Kevin?
Thank you, Carl, and good afternoon, everyone. I'm happy to review our financial results for the second quarter and the first half of 2021 and to provide a revised financial guidance for the balance of the year. Let's start on Slide 13. You've seen in our press release this afternoon, our record Q2 revenues of $217.8 million represented 364% reported growth from Q2 2020. Our GAAP-based net income before the amount attributable to non-controlling interests was $134.3 million for the second quarter of 2021. Now turning to Slide 14. Adjusted EBITDA, a non-GAAP measure, was $164.7 million for Q2, compared to $17.5 million for Q2 2020 and $101.9 million in Q1 2021. This represents an 841% increase year-over-year and a 62% sequential quarterly increase from Q1.
Our EBITDA margin was 76% in the quarter, up from both the 37% in Q2 2020 and the most recent 69% EBITDA margin in Q1 2021. The increase in adjusted EBITDA was primarily driven by overall sales volume increases and margin improvements from our Nucleic Acid Production business. On Slide 15, we present basic EPS, fully diluted EPS, and adjusted fully diluted EPS. Basic EPS is net income attributable to our Class A shares divided by the weighted average Class A shares. Fully diluted EPS equals net income prior to non-controlling interests divided by the weighted average for both Class A and B shares and other dilutive securities such as equity awards. Our adjusted fully diluted EPS equals adjusted net income divided by the weighted average of both Class A and B shares and other dilutive securities.
Coincidentally, both our basic and fully diluted EPS for the quarter were $0.44, while adjusted diluted EPS was also $0.44 per share. Moving to Slide 16. We continue to have an exceptionally strong balance sheet and adjusted free cash flows. Our cash and cash equivalents with our GAAP measures totaled $375 million at June 30th, 2021. Our strong EBITDA performance led to robust adjusted free cash flow for the quarter of $160.5 million. Adjusted free cash flow is a non-GAAP measure that we define as adjusted EBITDA less capital expenditures. With $547 million in long-term debt, $375 million in cash, and a trailing adjusted EBITDA of $389 million, we have a record low 0.4x net debt to adjusted EBITDA ratio and only 1.4x gross debt to adjusted EBITDA ratio.
This strong balance sheet and debt capacity allows us the financial flexibility to make both organic and inorganic investments that will drive innovation, capacity, address customer needs, and contribute to long-term growth. To provide some more insights into our business segment's financial performance for the quarter. Turning to Slide 17. As Carl mentioned earlier, our Nucleic Acid Production business fueled the most significant portion of the revenue growth for the first quarter. Nucleic Acid Production represented 88% of the company's total revenue in the quarter and generated $156.7 million in adjusted EBITDA in the quarter. The 81% adjusted EBITDA margin in this business is a record for Nucleic Acid Production and reflects increasing value of our unique products as well as the productivity gains and efficiencies from our state-of-the-art Wateridge manufacturing facility. I will tell you we're very pleased with our global supply chain and logistics efforts.
Under the leadership of our Vice President, Stephen McCusker, an industry veteran, we have substantially professionalized our efforts here. These efforts have resulted in supply chain agreements in place for our major raw material inputs, for which we have seen stable or even improved pricing based on volume increases. We have also diversified our supply chain to ensure we have multiple high-quality qualified vendors and geographical diversity. We also continue to improve our global logistics with our partners like FedEx and several best-in-class boutique freight partners that provide excellent logistics services. Our overall pricing, availability, diversification, quality, service levels, and on-time delivery metrics are all reviewed regularly and are trending favorably. CleanCap revenues from COVID-19 vaccine customers were approximately $156 million in the second quarter of 2021, a sequential increase of $65 million or 71% from Q1 2021. Our non-COVID-19 related Nucleic Acid Production revenue grew 11% sequentially.
Our Biologics Safety Testing business contributed 8% of the company's revenue in the second quarter. Our Cygnus-branded products, which comprise virtually all of the segment's business, grew to a record $18.2 million in the quarter, representing 3% growth over Q1 of 2021. This growth was driven by the increasing number of biologics and biosimilar drug development programs, as well as the new customers gained in the quarter attributed to the high quality and breadth of menu for our host cell protein ELISA kits. This included strong growth from our HEK kits used in vaccine and gene and cell therapy programs, as well as increasing contributions from our protein A leachate kits used to purify monoclonal antibodies and our endonuclease offerings. Further, we saw a strong quarter for our E. coli products used in many biosimilar programs. Our Biologics Safety Testing business delivered $14 million of adjusted EBITDA in the quarter.
Our protein detection business represented a smaller part of our overall business, accounting for only about 3% of revenues for the second quarter and about 2% of our adjusted EBITDA. As Carl has commented, we will be divesting this business to Thompson Street later this quarter. Corporate expenses that are not included in the segment adjusted EBITDA totals I just spoke of were $9.7 million in the quarter, relatively flat from the Q1 2021 levels of $10 million. Moving to slide 18 and our updated 2021 guidance. Today, we are raising our 2021 full-year revenue guidance to $745 million-$770 million, up from our prior guidance of $680 million-$720 million, a $58 million increase at the midpoint, even factoring in the removal of roughly $10 million for four months of protein detection revenues that was included in our previous full-year guidance.
Included in our overall total revenue range is our estimate for 2021 CleanCap revenues directly attributable to our COVID-19 vaccine customers, which we are estimating at $490 million-$510 million, up $45 million at the midpoint from our prior guidance. This total revenue guidance for the full year of 2021 reflects the expectation of mid-20% growth for the annual growth for our Biologics Safety Testing business. Coming off record last quarter for this business, we continue to see very solid market dynamics across this segment and geographies fueling growth in biologics. That, combined with new customer wins that build on the already strong base of repeat customers, is clearly supporting a more bullish outlook for this business segment in 2021. This guidance also reflects the divestiture of the Protein Detection business and the loss of that modest revenue contribution.
We see Protein Detection contributing approximately $18 million to Maravai on a reported basis in 2021 for the months of our actual ownership of this business, which we anticipate to be eight months. Given the relatively small contribution of Protein Detection to Maravai as a whole, we are not planning on presenting pro forma results with and without this segment. This updated guidance at the midpoint implies that our Nucleic Acid Production segment revenues will be around $670 million for 2021. Subtracting the midpoint of our COVID-19 CleanCap revenue guidance, you will see that our base nucleic acid business is shaping up to be roughly $170 million for the year. That would represent growth of roughly 60% versus the comparable total in 2020.
We continue to see good momentum and traction across our offerings here with strong CleanCap demand coming from outside of the major COVID-19 players as well, including initial orders for non-COVID vaccine development. Furthermore, our blue-chip customer base of gene and cell therapy companies represents an exciting mid to long-term opportunity as the validation of mRNA as a development platform is fueling rapid segment growth. We're extremely busy here, and we're very excited about our role as a key contributor to these new mRNA platforms for the foreseeable future. Turning to the quarterly gating of revenues for the rest of 2021, as we expected, our second quarter was incredibly strong, particularly in Nucleic Acid Production. At this stage, we see the second half of 2021 total revenues roughly evenly split between the third and fourth quarters.
Based on our total revenue guidance of $745 million-$770 million, that implies second half revenues of around $380 million-$400 million or around $190 million-$200 million per quarter, down slightly from the 2Q level of $218 million, but still reflecting second half revenue growth of over 6% at the midpoint of guidance for first half of 2021, even without the Protein Detection revenue contribution. As discussed on our last call, our revenue guidance is in large part based on our largest customers' rolling forecasts that extend out for several quarters and that are supported in the shorter term by binding POs, many of which go out several months.
On top of that, we have a forecasted funnel for our GMP suites that are used to mainly support builds for our customers' nucleic acid therapeutics programs. Based on these factors, our fiscal year 2021 revenue guidance comes with a considerable degree of poor visibility, will also be subject to some quarterly fluctuations. Based on these revenue expectations, we have updated our internal forecast and guidance for other key financial metrics. We expect our non-GAAP adjusted EBITDA to be in the range of $515 million-$535 million, which at the midpoint of that range represents growth of 207% and implied adjusted EBITDA margin of 69% at the midpoint of our 2021 revenue range. The full-year margin is moderated versus the most recently completed quarter that saw record CleanCap revenue contributions.
Furthermore, as Carl mentioned, we are continuing to look to make organic investments in our R&D and commercial organizations, and we continue to expand our employee base to meet record customer demand. Adjusted fully diluted EPS, a non-GAAP measure, is expected to be in the range of $1.30-$1.36 per share. This increase in our guidance here is directly tied to our revenue growth and overall projected full-year margin expansion on the heels of our strong second quarter results. As with our updated total revenue guidance, we anticipate the adjusted EPS for the third and fourth quarter to be relatively even, implying EPS for each quarter of around $0.34 per share at the midpoint of this guidance range. Turning to slide 19.
Adjusted fully diluted EPS is based on the assumption that all Class B shares are converted to Class A shares, resulting in a forecasted fully diluted share count of 260 million shares for the full year. The net income included in the adjusted fully diluted EPS has been adjusted to eliminate any net income or loss attributable to non-controlling interests as a result of the assumed full conversion of Class B shares for Class A shares. Additionally, our adjusted fully diluted EPS, including certain adjustments that do not reflect our core operations, are based on adjusted effective tax rate range of 23%-24%. The effective tax rate reflecting some forecasted improvement based on the geographical distribution of our growing revenue base. As it relates to certain other adjustments needed to get to our non-GAAP adjusted EBITDA range, we see the following items in 2021.
Interest expense of between $33 million and $35 million, depreciation and amortization also between $29 million and $32 million, adjusted tax rate of between 23% and 24%, equity-based compensation, which we show as a reconciling item from GAAP to non-GAAP, to be $10 million-$12 million in 2021. For 2021, we also expect to invest approximately $15 million-$25 million for capital expenditures or around 3% of total revenues. We will be further evaluating this total and associated spending timeline over the next quarter, given our commitment to the two new facilities that will come online mid-year 2022. At this stage, we estimate the overall capital for those new facilities to be roughly $25 million spread out over the next four quarters. Our reconciliation of net income to GAAP EBITDA and from GAAP EBITDA to adjusted EBITDA is presented in our press release that we issued earlier today.
Our segment-related information will be detailed in our Form 10-Q, which we plan on filing in the coming days. It was a very strong financial quarter for Maravai, by just about every metric, our business has never been stronger. I'm very excited about the increasing demand and expanding applications for our unique products, which demonstrate the value they hold for our customers. The commitment to fund even more innovation and capacity via new facilities and growing our human capital is clear evidence of how we're investing in the future via strategically applying our strong cash flow while also focusing our business on our highest growth potential markets. I'll turn the call back to Carl for some final remarks.
Well, thanks, Kevin. To wrap up, we had an incredibly strong first half of 2021 in our first calendar year as a public company. We feel great about the momentum we're seeing across our business, and we're pleased to be adjusting our guidance upwards significantly to reflect stronger demand expectations persisting for the remainder of the year and beyond. From COVID-19 vaccines to vaccines for influenza, malaria, and TB, to cell and gene therapies battling cancer, the transformative impact mRNA will have on global human health is only accelerating. We at Maravai are proud of the key role that our customers, partners, and employees are playing in making that happen. We will continue to focus on operational excellence, innovation, and people as our three strategic pillars for generating above-market growth. I'd now like to turn the call back over to Alexander to open the line for your questions. Alexander?
Thank you. At this time, I would like to remind everyone, in order to ask a question, please press star one on your telephone keypad. Again, that is star one to ask a question. We have your first question from Matt Sykes with Goldman Sachs. Your line is open.
Hi, good afternoon, everybody, and congrats on the quarter.
Thanks, Matt. We sure appreciate that.
No problem. Just kind of a cleanup question. Thank you very much for providing that detail on the progress you've made on the supply agreements. I'm sorry if I missed it, but did you break that out in between COVID and non-COVID in terms of what those supply agreements, what the mix of is? Are you going to break that out?
We did not. The majority would be non-COVID.
Okay, great. I mean, obviously some great progress that you've made and the net debt to EBITDA is pretty impressive. Just as you look across the space and you balance organic versus inorganic, and you look at the valuations on the inorganic side, can you accomplish what you want to accomplish part ways organically? Are there really some areas that you really want to add on to inorganically, and you'll just have to deal with the valuations as they are in the market today?
Well, Kev, do you want to take a shot at that or?
Yeah, sure, Matt. Look, I think we see some nice opportunities. Again, some of the assets that we're looking at are certainly things that are very close to what we've done historically. From that perspective, I think we have a little bit of a unique opportunity, and try to do so in manners that make a lot of sense financially, but also are very high quality as far as the quality of the products. There's certain things that we look at that don't exactly pass our tests when we do our diligence as well, and certainly keep that in mind. I think that we do have the flexibility, the know-how, the leadership, I would say, to do a lot of things organically, you see that with the investment in our capacity expansion.
We do have a lot of opportunities from our existing customer base on things that we are not precluded from an intellectual property perspective. That will be something we can control. Certainly, valuations are at the top end of historical ranges, but from our perspective, again, we're looking at things that are unique to our customers, might bring added capabilities or intellectual property access if we need them, or accelerate our ability to provide more of those products to our customers in a manner that might be much quicker than trying to do it organically. It's a balance, but I think we can execute both, and we're very active, as you can see, in both things here in focusing on organic growth and investment, as well as several different opportunities inorganically that we think would make a difference to our customers and our overall offering.
Great. Just one last question. When you issued the release on the Chula Vaccine Research Center relationship, you mentioned that you were having talks with other folks in the APAC region. Is that an area of an expansion for you, and are you making good progress there, COVID and maybe even non-COVID, as you kind of expand?
Yeah, it definitely is, we're seeing more and more opportunities come up, I think particularly in the COVID area as different countries are making their own investments similar, say, to Operation Warp Speed here in the U.S. We do expect that trend to continue. We also continue to see very strong growth in Biologics Safety Testing in Asia Pac, particularly both in the China market, Korea, and to a lesser degree, in Japan.
Yeah, Matt.
Thanks very much.
Yeah, just for your information, sales in Asia Pac were about 14% of revenues for this most recent quarter. China was about 3.5% of revenues for the most recent quarter.
Great. Thanks.
Yep.
We have your next question from Tejas Savant with Morgan Stanley. Your line's open.
Hey, Carl and team. Good afternoon. Just to kick things off, Carl, I hear your comments on the Delta variant and the building consensus for the need for boosters here. Given the vaccine hesitancy we've run into here in the U.S. and to a certain degree in pockets in Europe, are you seeing that start to filter through in any way in terms of the orders at all from Pfizer?
No. We see no diminution of their ordering pattern or the statements about future demand. As I mentioned, Pfizer specifically is talking about increasing their capacity by 1 billion more doses or 33% compared to what they expect to finish this year at.
Got it. Helpful. On a somewhat related note, do you have any visibility on whether you'd be specced into their flu vaccine? I think they're starting first human trials at some point in the third quarter. Sort of similar note here, on the other biopharma customers you mentioned as investing aggressively in mRNA development programs, any sort of early read on just showing up and winning that business?
Yeah. Look, on the first point, I'm constrained by confidentiality as to what I can say about my customers' programs if they haven't said anything about it. I'm limited in how I can respond to that. Suffice it to say that the platform that is being used by Pfizer and BioNTech for their major programs has been proven in the COVID-19 vaccines, and it would be extremely unusual to make a change in the 11th hour, especially on a program that you were trying to rapidly accelerate through regulatory approval. I think it's fair to say that we're in a good position there. Let's just leave it at that.
On the expanding demand for mRNA, either components themselves or for the molecules themselves, we have seen an incredible level of activity in our commercial organization. I think it's fair to say that all of the companies whose names are being bandied about as wanting to expand in the field have at some point or another given us a call.
Very helpful. Then one final one on the vaccinia capping enzyme announcement from Aldevron this morning. They talked about sort of a 10-fold increase in production efficiency for VCE. In your opinion, does this make enzymatic a more competitive alternative to CleanCap, although you do have a simpler workflow here? On a related note, just given the purchase by Danaher here, do you expect any shift in competitive dynamics to the degree that you overlap with them?
Yeah. That's an interesting question. When I finally saw the press release you were referring to this morning, actually, I think it was from Ginkgo Bioworks about their relationship with Aldevron. I have to admit that I wondered what all the fuss was about. If from Ginkgo, which I think is still a private company, for a little while yet, that's projecting what something like $150 million in total revenue in 2021. What they basically said is that they helped one customer, Aldevron, to scale up one manufacturing process for one enzyme by 10x. There was no data in the release that I saw on the quality or the performance of the enzymes in question, either before or after the scale up.
There was no indication that the scale difference would actually expand total capacity in the industry given the scarcity of some of the needed raw materials that are out there. There was certainly no claim made that this would significantly reduce costs for Aldevron. I'm hard pressed to see how that statement is much more than a press release from a young company accustomed to trying to get attention. I don't really know what to make of it. With respect to the competitive dynamics post the Danaher acquisition, we have an immense amount of respect for Danaher and the way that the DBS is applied to all of their areas, including sales and marketing. We will view them as a competitor and a supplier, just like many other people are in the industry.
Fair enough. Congrats on the good quarter here, guys. Thank you.
Well, thanks so much.
We have your next question from Dan Arias with Stifel. Your line's open.
Afternoon, guys. Thanks for the questions. Carl, maybe just a general one on a level of visibility that you have on orders coming in from the mRNA vaccine guys. Have you been able to gain additional insight from your supply conversations with them when it comes to just whether or not there's stocking going on for a booster or pediatric usage or expansion into the third world? Do orders generally look the same as other orders the way that they did earlier on in the process?
Yeah, that's interesting, Dan. I guess what I would say is that we haven't seen. Let me stop and say it differently. Those issues, and the increased demand possible for each of those initiatives you mentioned have been discussed by our customers with us as they're thinking about their forecasting. They have, I think resulted in clearer 12-month out forecasts of what their expectations are. To us, they reflect the kind of public statements that are being made that we have to expand our capacity, say, by a third in the case of the Pfizer program. I think all of that is going in the right direction, and we're not seeing anybody back off in any way, if that's what you're getting at.
Yeah, that's helpful. I guess I was just trying to just understand whether you have sort of a line of sight in what the specifics of what Pfizer or another party might be trying to do, but I think I get your point there. Okay. Then maybe just secondly, I guess a bit of a technical question, but nothing crazy. As we start to think about expanded usage beyond COVID vaccines and the investment that you referenced, should we consider the performance benefits of CleanCap kind of being similar to what you're experiencing today when you compare legacy or alternative capping approaches to yours? In other words, I think there was like a 3x benefit on yield, and the cost was a third of legacy methods when we were going through the process. Is that generally translatable across the board when you think about some of these non-COVID projects?
I think any one program or another can have significant differences in what they need to consume in the way of components just based on the process or methods that they're using. I wouldn't say it'll always be static. I think that it's fair to say that the benefits that CleanCap has versus enzymatic capping are similar across multiple different programs, whether for vaccines or therapeutics. We're in the process of working out a finishing a paper that'll be submitted for publication that shows our experience with these various methods and shows some of those benefits which probably will be released sometime later this year.
Okay. Very helpful. Thank you.
You bet, Dan. Thanks.
We have your next question from Matt Larew with William Blair. Your line is open.
Yeah, good afternoon. You've obviously discussed the great visibility you have into your existing business. Just curious in terms of the new facility in San Diego, you mentioned how much capacity has been increased in the biologic safety testing expansion, but how much is capacity in total increasing with the investment in San Diego, and how much visibility do you have in terms of soaking up that capacity you're building out?
Yeah, Matt, I don't think I can give you a numerical answer to that yet. It's still a little bit early, think of almost the new facility here in San Diego as being overflow for us. The functions that we move out of our Wateridge facility here will then free up additional space that will be readily converted to additional manufacturing capacity. We will have these other support functions, laboratories, innovation centers, as well as some limited pilot plant manufacturing capacity in the new facility. Yeah, probably a little bit too early to tell you until we've scoped it all out and got it down on a piece of paper, but I'd say we may be able to comment more next quarter.
Okay, fair enough, Carl. Kevin, just on the gross margin, obviously, very strong end of the quarter, and the Protein Detection business you're divesting there has a little bit lower margin. Just wanted to know kind of an update of thinking around the gross margin line long term. You alluded today to some additional OpEx spending specific on R&D.
Yeah.
But-
Yeah, that would be R&D. I mean, our gross margins are very strong, as you noted, and should continue to be so. Certainly in the second quarter, we had a record production level of output. We sort of, to some degree, challenged ourselves to see what we could do within the quarter, just from a production perspective. I think that was a very good experience for us. We did a record level of output, and it certainly supports some of the previous comments we made with how we could annualize this business to north of $1 billion of output out of San Diego.
You layer in, I think, the additional ability to expand that as we were just talking to with the last question, as we kind of move out some of the other research and other areas here to expand what we do here just for pure production. We feel really good about that. The additional operating costs that'll hit the COGS line for these new facilities isn't much. We're talking probably about $5 million on an annualized basis for Nucleic Acid Production and maybe a million and a half for the new facility for Biologics Safety Testing. Those things will not have a huge impact on the gross margin going forward. As I spoke to, we feel really good about commodity costs right now. Our supply chain team's doing a great job.
To the extent the mix stays relatively the same, we're going to continue to see strong margins, certainly benefited a little bit by the high revenues in the second quarter and the unique production that we produced. Overall, very stable margins going forward on the gross margin line with just some of those added costs, which are going to be pretty small.
Okay. Congrats on the quarter.
Thank you.
Thanks. We appreciate it.
We have your next question from Katie Trychin with Credit Suisse. Your line's open.
Hi. Thanks for taking my question. On the plasmid DNA side, can you provide an update on how that is ramping or tracking relative to expectations? Is it still largely used to support internal operations, or have you begun marketing that more so to customers? We've seen several investments there on the plasmid DNA landscape over the past year. How are you thinking about your competitive positioning there and expectations on investment going forward? Thanks.
Yeah. Thanks for the question, Katie. Yes, the program is tracking to the initial expectations. We'll see how we finish the year here over the next four or five months. Our focus is, as you suggested, entirely on supporting our mRNA manufacturing customers so that we can provide them plasmids quicker than they can get them in the open market. To the degree that people are putting in plasmid capacity for general competition, say with Aldevron, we don't really see that directly affecting us, although it certainly will ultimately make it easier for all of our customers, joint customers, to get access to plasmids quickly and reliably, which is what this is all about.
Okay, great. Maybe on the M&A side, just digging in a little deeper, can you speak to some of the opportunities that you're looking at in the market? What are your target focus areas, and what would the sweet spot be in terms of sizing? Thanks.
Look, obviously, we will be focused entirely now on Nucleic Acid Production, Biologics Safety Testing, as areas of investment, and they are both important. I would say that we see more specific opportunities in nucleic acid testing because of the nature of the industry and the complex supply chains involved, et cetera. We're actively looking, as we have always been, at opportunities there. You will see some that are focused on supply chain rationalization and securitization, if that's a word, for us. Over time, you'll also see us expanding into other parts or other technologies that are used in the delivery of cell and gene therapies as part of a natural vertical expansion. Those are the areas, generally speaking. We don't really have any size limitations or preconceived notions. Certainly anything that would be considered a transformative acquisition is handled or evaluated on a one-off basis.
You can never anticipate those, and they have generally relatively low probability of coming about. We actively scan anybody who's got positive EBITDA and a sales track record, and we're willing to do deals for small companies and technologies that have promise all the way through to somebody who has anywhere from $50,000 to hundreds of millions of dollars of EBITDA.
Okay. That's great. Thanks much.
Thank you.
We have your next question from Michael Ryskin with Bank of America. Your line is open.
Thanks for taking the question, guys. Two quick ones, hopefully. One on the updated guide for 2021 and sort of the outlook for the second half. We talked about a number of times in the past of your visibility in terms of POs and some of the bigger customers. If we look forward from 2Q, especially on the COVID CleanCap side of things, it does imply a little bit of a slowdown as you go into the second half of the year. Just wondering sort of, could you remind us, what are you building in, what are you not building in? Is it sort of just the immediate POs that you have on hand? Sort of how much conservatism is in that outlook? I've got a follow-up.
Yeah.
Look, I think there is some natural timing differences with our forecast heading into the year. We always didn't see it going completely flat, not just based on what our customers are asking us to do for them and when they need the product. Look, I think at this stage, I think we continue to see a pretty steady state moving forward. I know it's a little bit down from the peak we saw here in the second quarter. That can always change as well. This is changing pretty regularly, and we pretty much have new information every month as we do our S&OP process and roll up our forward-looking demand. Certainly as Carl mentioned, this is a pretty steady state. Our customers are making pretty bold statements with regards to increasing their production next year.
We're looking forward in our lens four to six quarters forward, feel real good about what we see, feel real good about our capacity and some of the investments that we need to continue to deliver on increasing revenues. I think that as we sit here today, I think we have a real solid book of what we see now versus what we saw three months ago when we last talked about our guidance. That's why we're taking it up meaningfully at the midpoint for both COVID and non-COVID related revenues, as well as increasing it in contemplation of losing off $10 million of the protein detection business revenues as well. A little bit of choppiness, certainly, but still really strong outlook for the remainder of the year.
On that point, could you remind me, I'm sure it varies customer by customer and project by project, but what sort of a standard lead time we should think of from when you recognize revenues for CleanCap versus when the end customer's got vaccine shipping out the door?
Well, I think the best way to think about that is this is very much a real-time operation, so there's really not much inventory in the pipeline. When we finish product and ship it, we believe that it goes into the customer's QA/QC testing right away to prepare it for use. Our customers have disclosed at various points in time that they're running sort of 120-day cycle time and endeavoring to reduce that as much as they can. That should give you a feel that it's probably in somebody's arm three or four months later.
Okay, thanks. One last quick one if I could squeeze it in. On the Protein Detection divestment, just curious if you could give us a little more insight into how that process came about. Was it something you'd been thinking about for some time, just going back to the timing of the IPO, still relatively recent, or was it sort of the right opportunity came up and you reevaluated investment needs elsewhere and decided to sort of pull the trigger?
No, we were approached by Thompson Street about their interest in the field. They were credible because they've made other investments in immunohistochemistry. We gave them access to some limited amounts of information, and they stepped forward with what we thought was a fair valuation.
Okay. Thanks so much.
Alexander, we have time for I think one more question.
Thank you. We have your next question from Catherine Schulte with Baird. Your line is open.
Hey, guys. Thanks for the question. I guess I just wanted to go to your comment on ramping your R&D over the next several years, just in terms of programs getting the most attention, I guess how much is improving and expanding upon applications for CleanCap and other existing products versus pursuing new product categories and what might those new product opportunities look like?
Well, great question. We have several lines of investment and innovation going on in CleanCap simultaneously. They relate to other constructs, or they relate to modifications of the broader mRNA molecule produced with CleanCap that can affect its actual translation into proteins in the body and the success of those proteins in doing what they're intended to do. Multiple threads exist there. Another area of innovation for us is really in process development as it pertains to CleanCap. How can we produce larger and larger quantities of this more efficiently and more consistently with fewer steps? That's a big focus for us right now, both upstream and downstream, from where we actually utilize the CleanCap molecules.
Beyond that, there's a lot of innovation that happens in our normal nucleic acid business, independent of CleanCap or maybe with CleanCap incorporated in it, where we're innovating with our customers on what molecules can actually be synthesized successfully, how can they be utilized, and how do you modify something that hasn't worked quite as you initially intended. There's really a number of those things that are underway. We're also evaluating technologies that can be used for manufacturing alternative technologies and in some cases, some really innovative platforms that may extend the reach of our manufacturing capacity and move it closer towards patient care settings where it's important to rapidly be able to synthesize individualized therapies. I'd say those are probably the three major areas.
All right, great. Thank you.
All right. Thanks, Catherine. With that, Alexander, we'll turn it back to you.
I'm showing no further questions at this time. Ms. Deb Hart, please continue.
Well, thanks everyone for joining us today. Just a note to please check out our events website. We'll be presenting at several conferences during the month of September. Feel free to call me if you have any questions, and we hope you have a great evening.
Ladies and gentlemen, this concludes today's conference call. Thank you for your participation. You may now disconnect.